ArticlePlant molecular biology2006
Expression of bacterial L-aspartate-alpha-decarboxylase in tobacco increases beta-alanine and pantothenate levels and improves thermotolerance.
Article in Plant molecular biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 42 citations in OpenAlex.
- β-Alanine Is an Unexploited Neurotransmitter in the Pathogenesis and Treatment of Alzheimer's Disease.NeuroSci · 2026Review
- Development of probiotic E. coli Nissle 1917 for β-alanine production by using protein and metabolic engineering.Applied microbiology and biotechnology · 2023Article
- NMR as a "Gold Standard" Method in Drug Design and Discovery.Molecules (Basel, Switzerland) · 2020Review
- Substrate inactivation of bacterial L-aspartate α-decarboxylase from Corynebacterium jeikeium K411 and improvement of molecular stability by saturation mutagenesis.World journal of microbiology & biotechnology · 2019Article
- The Synthesis and Role of β-Alanine in Plants.Frontiers in plant science · 2019Review
- Primary Metabolism, Phenylpropanoids and Antioxidant Pathways Are Regulated in Potato as a Response to Potato virus Y Infection.PloS one · 2016Article
- Overexpression of Rice Glutaredoxin OsGrx_C7 and OsGrx_C2.1 Reduces Intracellular Arsenic Accumulation and Increases Tolerance in Arabidopsis thaliana.Frontiers in plant science · 2016Article
- Aspartate Decarboxylase is Required for a Normal Pupa Pigmentation Pattern in the Silkworm, Bombyx mori.Scientific reports · 2015Article
- Some like it hot, some like it warm: phenotyping to explore thermotolerance diversity.Plant science : an international journal of experimental plant biology · 2012Review
- An activator for pyruvoyl-dependent l-aspartate α-decarboxylase is conserved in a small group of the γ-proteobacteria including Escherichia coli.MicrobiologyOpen · 2012Article
- Aldehyde Dehydrogenases in Arabidopsis thaliana: Biochemical Requirements, Metabolic Pathways, and Functional Analysis.Frontiers in plant science · 2011Article
- Article
- Transplastomic expression of bacterial L-aspartate-alpha-decarboxylase enhances photosynthesis and biomass production in response to high temperature stress.Transgenic research · 2009Article
- Improper excess light energy dissipation in Arabidopsis results in a metabolic reprogramming.BMC plant biology · 2009Article
- Towards engineering increased pantothenate (vitamin B(5)) levels in plants.Plant molecular biology · 2008Article
- Pantothenate synthetase is essential but not limiting for pantothenate biosynthesis in Arabidopsis.Plant molecular biology · 2008Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
L- Aspartate-alpha-decarboxylase catalyzes the decarboxylation of L -aspartate to generate Beta-alanine and carbon dioxide. This is an unusual pyruvoyl-dependent enzyme unique to prokaryotes that undergoes limited self-processing. The Escherichia coli pan D gene encoding L- aspartate-alpha-decarboxylase was expressed under a constitutive promoter in transgenic tobacco. Transgene expression was verified by assays based on RNA blots, immunoblots and enzyme activity in vitro. The pan D lines had increased levels of leaf Beta-alanine (1.2- to 4-fold), pantothenate (3.2- to 4.1-fold) and total free amino acids (up to 3.7-fold) compared to wild-type and vector controls. Growth of homozygous lines expressing E. coli L- aspartate-alpha-decarboxylase was less affected than that of the control lines when the plants were stressed for 1 week at 35 degrees C. When transferred from 35 to 30 degrees C for 3 weeks, the Pan D transgenic lines recovered significantly (P <or= 0.001) better than the controls: Pan D lines had on an average 54% and 84% greater fresh and dry weights respectively, compared to the controls. Homozygous lines expressing E. coli L- aspartate-alpha-decarboxylase had significantly greater thermotolerance (P<or=0.05) during germination. At 42 degrees C, 95% of two T3 Pan D transgenic line seeds germinated after 12 days compared to 73% for the wild-type seeds. Our results indicated that E. coli L-aspartate-alpha-decarboxylase was correctly processed and active in the transgenic eukaryotic host and its expression resulted in increased thermotolerance in tobacco.
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