ReviewMicrobiology and molecular biology reviews : MMBR2006
Structure-function relationships of glucansucrase and fructansucrase enzymes from lactic acid bacteria.
Review in Microbiology and molecular biology reviews : MMBR, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 104 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
104 citing papers in PubMed, 444 citations in OpenAlex.
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- Compositional and Functional Characterization of Glycolipid Biosurfactants from an Olive-DerivedJournal of microbiology and biotechnology · 2026Article
- Screening and optimisation of crude exopolysaccharide production by marine Brevibacterium sp. JAB08 isolated from the jellyfish Chrysaora sp. from the coastal waters of Goa.Antonie van Leeuwenhoek · 2026Article
- Patchouli Alcohol as a Potential Anti-Cariogenic Compound TargetingMolecules (Basel, Switzerland) · 2026Article
- Fecal Material of Captive Wild Animals as Source of CAZymes With Application Potential.Chembiochem : a European journal of chemical biology · 2026Article
- AJournal of bacteriology · 2026Review
- Apilactobacillus kunkeei as a promising fermentation candidate for reducing free sugars in fruit juices.BMC microbiology · 2026Article
- Reproducible 3D bioprinting ofMicrobiology spectrum · 2025Article
- Cold induced expression of a novel levansucrase gene sacB1 enhances exopolysaccharide production and stress resilience in Leuconostoc mesenteroides.Scientific reports · 2025Article
- Insight of Metal Ions in Enzymatic Synthesis of Levan: The Metal-Binding Loop.Journal of microbiology and biotechnology · 2025Article
- Tailoring the natural rare sugars D-tagatose and L-sorbose to produce novel functional carbohydrates.NPJ science of food · 2024Article
- Exploring the Diversity and Potential Use of Flower-Derived Lactic Acid Bacteria in Plant-Based Fermentation: Insights into Exo-Cellular Polysaccharide Production.Foods (Basel, Switzerland) · 2024Article
- The Discovery, Molecular Cloning, and Characterization of DextransucraseMolecules (Basel, Switzerland) · 2024Article
- Biofilm formation by agave epiphytic lactic acid bacteria fed with agave fructans.World journal of microbiology & biotechnology · 2023Article
- Recent Progress in 1,2-Molecules (Basel, Switzerland) · 2023Review
- Fermented Whey Ewe's Milk-Based Fruit Smoothies: Bio-Recycling and Enrichment of Phenolic Compounds and Improvement of Protein Digestibility and Antioxidant Activity.Antioxidants (Basel, Switzerland) · 2023Article
- Glycosyltransferases Expression Changes inFoods (Basel, Switzerland) · 2023Article
- In Situ Raman Analysis of Biofilm Exopolysaccharides Formed inInternational journal of molecular sciences · 2023Article
- Dietary Supplementation of Microbial Dextran and Inulin Exerts Hypocholesterolemic Effects and Modulates Gut Microbiota in BALB/c Mice Models.International journal of molecular sciences · 2023Article
44 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lactic acid bacteria (LAB) employ sucrase-type enzymes to convert sucrose into homopolysaccharides consisting of either glucosyl units (glucans) or fructosyl units (fructans). The enzymes involved are labeled glucansucrases (GS) and fructansucrases (FS), respectively. The available molecular, biochemical, and structural information on sucrase genes and enzymes from various LAB and their fructan and alpha-glucan products is reviewed. The GS and FS enzymes are both glycoside hydrolase enzymes that act on the same substrate (sucrose) and catalyze (retaining) transglycosylation reactions that result in polysaccharide formation, but they possess completely different protein structures. GS enzymes (family GH70) are large multidomain proteins that occur exclusively in LAB. Their catalytic domain displays clear secondary-structure similarity with alpha-amylase enzymes (family GH13), with a predicted permuted (beta/alpha)(8) barrel structure for which detailed structural and mechanistic information is available. Emphasis now is on identification of residues and regions important for GS enzyme activity and product specificity (synthesis of alpha-glucans differing in glycosidic linkage type, degree and type of branching, glucan molecular mass, and solubility). FS enzymes (family GH68) occur in both gram-negative and gram-positive bacteria and synthesize beta-fructan polymers with either beta-(2-->6) (inulin) or beta-(2-->1) (levan) glycosidic bonds. Recently, the first high-resolution three-dimensional structures have become available for FS (levansucrase) proteins, revealing a rare five-bladed beta-propeller structure with a deep, negatively charged central pocket. Although these structures have provided detailed mechanistic insights, the structural features in FS enzymes dictating the synthesis of either beta-(2-->6) or beta-(2-->1) linkages, degree and type of branching, and fructan molecular mass remain to be identified.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.