ArticleEukaryotic cell2006
Mutational analysis of the glycosylphosphatidylinositol (GPI) anchor pathway demonstrates that GPI-anchored proteins are required for cell wall biogenesis and normal hyphal growth in Neurospora crassa.
Article in Eukaryotic cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 77 citations in OpenAlex.
- Molecular Insights into Fungal Glycosylphosphatidylinositol Transamidase Complex.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Growth conditions shape the proteome and diversity ofCell surface (Amsterdam, Netherlands) · 2025Article
- Stage-specific RNA regulomes ofIMA fungus · 2025Article
- Extracellular targeting of Neurospora crassa cell wall and secreted glycoproteins by DFG-5.Fungal genetics and biology : FG & B · 2022Article
- Molecular characterization of stress tolerance genes associated with D. indicus strain under extreme environment conditions.Environmental geochemistry and health · 2021Article
- Unveiling the structure of GPI-anchored protein of Malassezia globosa and its pathogenic role in pityriasis versicolor.Journal of molecular modeling · 2021Article
- The Glycosylphosphatidylinositol-Anchored Superoxide Dismutase ofJournal of fungi (Basel, Switzerland) · 2021Article
- Quantitative Proteome Profiling Reveals Cellobiose-Dependent Protein Processing and Export Pathways for the Lignocellulolytic Response in Neurospora crassa.Applied and environmental microbiology · 2020Article
- The Genetics and Biochemistry of Cell Wall Structure and Synthesis inFrontiers in microbiology · 2019Review
- Identification and manipulation ofBiotechnology for biofuels · 2019Article
- Disruption of gul-1 decreased the culture viscosity and improved protein secretion in the filamentous fungus Neurospora crassa.Microbial cell factories · 2018Article
- De novo assembly and comparative transcriptome analysis of Monilinia fructicola, Monilinia laxa and Monilinia fructigena, the causal agents of brown rot on stone fruits.BMC genomics · 2018Article
- Disruption of calcineurin catalytic subunit (cnaA) in Epichloë festucae induces symbiotic defects and intrahyphal hyphae formation.Molecular plant pathology · 2018Article
- Mutation of the Slt2 ortholog from Cryphonectria parasitica results in abnormal cell wall integrity and sectorization with impaired pathogenicity.Scientific reports · 2017Article
- Genetic and biochemical characterization of the GH72 family of cell wall transglycosylases in Neurospora crassa.Fungal genetics and biology : FG & B · 2017Article
- A proteomic and genetic analysis of the Neurospora crassa conidia cell wall proteins identifies two glycosyl hydrolases involved in cell wall remodeling.Fungal genetics and biology : FG & B · 2016Article
- Characterization of the Sclerotinia sclerotiorum cell wall proteome.Molecular plant pathology · 2016Article
- Hyphal ontogeny inF1000Research · 2016Review
- The Neurospora crassa CPS-1 polysaccharide synthase functions in cell wall biosynthesis.Fungal genetics and biology : FG & B · 2014Article
- Early colony establishment in Neurospora crassa requires a MAP kinase regulatory network.Genetics · 2013Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using mutational and proteomic approaches, we have demonstrated the importance of the glycosylphosphatidylinositol (GPI) anchor pathway for cell wall synthesis and integrity and for the overall morphology of the filamentous fungus Neurospora crassa. Mutants affected in the gpig-1, gpip-1, gpip-2, gpip-3, and gpit-1 genes, which encode components of the N. crassa GPI anchor biosynthetic pathway, have been characterized. GPI anchor mutants exhibit colonial morphologies, significantly reduced rates of growth, altered hyphal growth patterns, considerable cellular lysis, and an abnormal "cell-within-a-cell" phenotype. The mutants are deficient in the production of GPI-anchored proteins, verifying the requirement of each altered gene for the process of GPI-anchoring. The mutant cell walls are abnormally weak, contain reduced amounts of protein, and have an altered carbohydrate composition. The mutant cell walls lack a number of GPI-anchored proteins, putatively involved in cell wall biogenesis and remodeling. From these studies, we conclude that the GPI anchor pathway is critical for proper cell wall structure and function in N. crassa.
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