ArticleEukaryotic cell2006
The actin gene ACT1 is required for phagocytosis, motility, and cell separation of Tetrahymena thermophila.
Article in Eukaryotic cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 46 citations in OpenAlex.
- Distinct macrophage uptake of engineered and biological particles driven by host age and sex.Science and technology of advanced materials · 2026Article
- Importin-9 and a TPR domain protein MpH drive periodic patterning of ciliary arrays in Tetrahymena.The Journal of cell biology · 2025Article
- Close cooperation between Semi1 and Semi2 proteins is essential for pronuclear positioning inMolecular biology of the cell · 2025Article
- Global and local functions of the Fused kinase ortholog CdaH in intracellular patterning in Tetrahymena.Journal of cell science · 2024Article
- Serotonin signaling regulates actomyosin contractility during morphogenesis in evolutionarily divergent lineages.Nature communications · 2023Article
- Basal body organization and cell geometry during the cell cycle inMolecular biology of the cell · 2023Article
- Intracellular connections between basal bodies promote the coordinated behavior of motile cilia.Molecular biology of the cell · 2022Article
- Anterior-posterior pattern formation in ciliates.The Journal of eukaryotic microbiology · 2022Review
- Ciliate cortical organization and dynamics for cell motility: Comparing ciliates and vertebrates.The Journal of eukaryotic microbiology · 2022Review
- Transcriptomic Differences between Free-Living and ParasiticMicroorganisms · 2022Article
- Ccdc113/Ccdc96 complex, a novel regulator of ciliary beating that connects radial spoke 3 to dynein g and the nexin link.PLoS genetics · 2021Article
- Shulin packages axonemal outer dynein arms for ciliary targeting.Science (New York, N.Y.) · 2021Article
- Who Needs a Contractile Actomyosin Ring? The Plethora of Alternative Ways to Divide a Protozoan Parasite.Frontiers in cellular and infection microbiology · 2019Review
- Multiple essential functions of Plasmodium falciparum actin-1 during malaria blood-stage development.BMC biology · 2017Article
- Tetrahymena thermophila: a divergent perspective on membrane traffic.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2014Review
- Antibody screening identifies 78 putative host proteins involved in Cyprinid herpesvirus 3 infection or propagation in common carp, Cyprinus carpio L.Journal of fish diseases · 2013Article
- ADF/cofilin is not essential but is critically important for actin activities during phagocytosis in Tetrahymena thermophila.Eukaryotic cell · 2013Article
- Mass spectrometry imaging of freeze-dried membrane phospholipids of dividing Tetrahymena pyriformis.Surface and interface analysis : SIA · 2013Article
- Conservation and innovation in Tetrahymena membrane traffic: proteins, lipids, and compartments.Methods in cell biology · 2012Review
- CCTalpha and CCTdelta chaperonin subunits are essential and required for cilia assembly and maintenance in Tetrahymena.PloS one · 2010Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
A previously identified Tetrahymena thermophila actin gene (C. G. Cupples and R. E. Pearlman, Proc. Natl. Acad. Sci. USA 83:5160-5164, 1986), here called ACT1, was disrupted by insertion of a neo3 cassette. Cells in which all expressed copies of this gene were disrupted exhibited intermittent and extremely slow motility and severely curtailed phagocytic uptake. Transformation of these cells with inducible genetic constructs that contained a normal ACT1 gene restored motility. Use of an epitope-tagged construct permitted visualization of Act1p in the isolated axonemes of these rescued cells. In ACT1Delta mutant cells, ultrastructural abnormalities of outer doublet microtubules were present in some of the axonemes. Nonetheless, these cells were still able to assemble cilia after deciliation. The nearly paralyzed ACT1Delta cells completed cleavage furrowing normally, but the presumptive daughter cells often failed to separate from one another and later became reintegrated. Clonal analysis revealed that the cell cycle length of the ACT1Delta cells was approximately double that of wild-type controls. Clones could nonetheless be maintained for up to 15 successive fissions, suggesting that the ACT1 gene is not essential for cell viability or growth. Examination of the cell cortex with monoclonal antibodies revealed that whereas elongation of ciliary rows and formation of oral structures were normal, the ciliary rows of reintegrated daughter cells became laterally displaced and sometimes rejoined indiscriminately across the former division furrow. We conclude that Act1p is required in Tetrahymena thermophila primarily for normal ciliary motility and for phagocytosis and secondarily for the final separation of daughter cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.