ArticleEukaryotic cell2006
Differential effects of heterochromatin protein 1 isoforms on mitotic chromosome distribution and growth in Dictyostelium discoideum.
Article in Eukaryotic cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 32 citations in OpenAlex.
- Review
- Nuclear Envelope Dynamics inCells · 2025Review
- Convergent pairs of highly transcribed genes restrict chromatin looping in Dictyostelium discoideum.Nucleic acids research · 2025Article
- Article
- TheCells · 2021Review
- Convergent evolution of integration site selection upstream of tRNA genes by yeast and amoeba retrotransposons.Nucleic acids research · 2018Article
- Regulation of nucleosome positioning by a CHD Type III chromatin remodeler and its relationship to developmental gene expression inGenome research · 2017Article
- Analysis of the Microprocessor in Dictyostelium: The Role of RbdB, a dsRNA Binding Protein.PLoS genetics · 2016Article
- Article
- Evolution of the nucleus.Current opinion in cell biology · 2014Review
- Functional impact of Aurora A-mediated phosphorylation of HP1γ at serine 83 during cell cycle progression.Epigenetics & chromatin · 2013Article
- Functional characterization of CP148, a novel key component for centrosome integrity in Dictyostelium.Cellular and molecular life sciences : CMLS · 2012Article
- Dynamics of a novel centromeric histone variant CenH3 reveals the evolutionary ancestral timing of centromere biogenesis.Nucleic acids research · 2010Article
- Dictyostelium discoideum CenB is a bona fide centrin essential for nuclear architecture and centrosome stability.Eukaryotic cell · 2009Article
- Balance between distinct HP1 family proteins controls heterochromatin assembly in fission yeast.Molecular and cellular biology · 2008Article
- Conifers have a unique small RNA silencing signature.RNA (New York, N.Y.) · 2008Article
- Cell cycle regulated transcription of heterochromatin in mammals vs. fission yeast: functional conservation or coincidence?Cell cycle (Georgetown, Tex.) · 2008Article
- Dictyostelium Aurora kinase has properties of both Aurora A and Aurora B kinases.Eukaryotic cell · 2008Article
- Structural and biochemical advances in mammalian RNAi.Journal of cellular biochemistry · 2006Review
- On the origin and functions of RNA-mediated silencing: from protists to man.Current genetics · 2006Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heterochromatin protein 1 (HP1) is a well-characterized heterochromatin component conserved from fission yeast to humans. We identified three HP1-like genes (hcpA, hcpB, and hcpC) in the Dictyostelium discoideum genome. Two of these (hcpA and hcpB) are expressed, and the proteins colocalized as green fluorescent protein (GFP) fusion proteins in one major cluster at the nuclear periphery that was also characterized by histone H3 lysine 9 dimethylation, a histone modification so far not described for Dictyostelium. The data strongly suggest that this cluster represents the centromeres. Both single-knockout strains displayed only subtle phenotypes, suggesting that both isoforms have largely overlapping functions. In contrast, disruption of both isoforms appeared to be lethal. Furthermore, overexpression of a C-terminally truncated form of HcpA resulted in phenotypically distinct growth defects that were characterized by a strong decrease in cell viability. Although genetic evidence implies functional redundancy, overexpression of GFP-HcpA, but not GFP-HcpB, caused growth defects that were accompanied by an increase in the frequency of atypic anaphase bridges. Our data indicate that Dictyostelium discoideum cells are sensitive to changes in HcpA and HcpB protein levels and that the two isoforms display different in vivo and in vitro affinities for each other. Since the RNA interference (RNAi) machinery is frequently involved in chromatin remodeling, we analyzed if knockouts of RNAi components influenced the localization of H3K9 dimethylation and HP1 isoforms in Dictyostelium. Interestingly, heterochromatin organization appeared to be independent of functional RNAi.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.