ArticleEukaryotic cell2006
Analysis of the phosphoproteome of Chlamydomonas reinhardtii provides new insights into various cellular pathways.
Article in Eukaryotic cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 70 citations in OpenAlex.
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- The phosphorylated redox proteome of Chlamydomonas reinhardtii: Revealing novel means for regulation of protein structure and function.Redox biology · 2018Article
- Evolution of the SOUL Heme-Binding Protein Superfamily Across Eukarya.Journal of molecular evolution · 2016Article
- The global phosphoproteome of Chlamydomonas reinhardtii reveals complex organellar phosphorylation in the flagella and thylakoid membrane.Molecular & cellular proteomics : MCP · 2014Article
- Article
- Whole genome sequencing identifies a deletion in protein phosphatase 2A that affects its stability and localization in Chlamydomonas reinhardtii.PLoS genetics · 2013Article
- The proteome of copper, iron, zinc, and manganese micronutrient deficiency in Chlamydomonas reinhardtii.Molecular & cellular proteomics : MCP · 2013Article
- The eukaryotic flagellum makes the day: novel and unforeseen roles uncovered after post-genomics and proteomics data.Current protein & peptide science · 2012Review
- Molecular pathways: the role of primary cilia in cancer progression and therapeutics with a focus on Hedgehog signaling.Clinical cancer research : an official journal of the American Association for Cancer Research · 2012Article
- Characterization of a subunit of the outer dynein arm docking complex necessary for correct flagellar assembly in Leishmania donovani.PLoS neglected tropical diseases · 2010Article
- Analysis of flagellar phosphoproteins from Chlamydomonas reinhardtii.Eukaryotic cell · 2009Article
- Identification of serine/threonine kinase substrates in the human pathogen group B streptococcus.Journal of proteome research · 2009Article
- Flexibility in anaerobic metabolism as revealed in a mutant of Chlamydomonas reinhardtii lacking hydrogenase activity.The Journal of biological chemistry · 2009Article
- The power of functional proteomics: Components of the green algal eyespot and its light signaling pathway(s).Plant signaling & behavior · 2008Article
- Rapidly alternating transmission mode electron-transfer dissociation and collisional activation for the characterization of polypeptide ions.Analytical chemistry · 2008Article
- The green yeast uses its plant-like clock to regulate its animal-like tail.Genes & development · 2008Article
- The phosphoproteome of a Chlamydomonas reinhardtii eyespot fraction includes key proteins of the light signaling pathway.Plant physiology · 2008Article
- Chlamydomonas outer arm dynein alters conformation in response to Ca2+.Molecular biology of the cell · 2007Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The unicellular flagellated green alga Chlamydomonas reinhardtii has emerged as a model organism for the study of a variety of cellular processes. Posttranslational control via protein phosphorylation plays a key role in signal transduction, regulation of gene expression, and control of metabolism. Thus, analysis of the phosphoproteome of C. reinhardtii can significantly enhance our understanding of various regulatory pathways. In this study, we have grown C. reinhardtii cultures in the presence of an inhibitor of Ser/Thr phosphatases to increase the phosphoprotein pool. Phosphopeptides from these cells were enriched by immobilized metal-ion affinity chromatography and analyzed by nano-liquid chromatography-electrospray ionization-mass spectrometry (MS) with MS-MS as well as neutral-loss-triggered MS-MS-MS spectra. In this way, we were able to identify 360 phosphopeptides from 328 different phosphoproteins of C. reinhardtii, thus providing new insights into a variety of cellular processes, including metabolic and signaling pathways. Comparative analysis of the phosphoproteome also yielded new functional information on proteins controlled by redox regulation (thioredoxin target proteins) and proteins of the chloroplast 70S ribosome, the centriole, and especially the flagella, for which 32 phosphoproteins were identified. The high yield of phosphoproteins of the latter correlates well with the presence of several flagellar kinases and indicates that phosphorylation/dephosphorylation represents one of the key regulatory mechanisms of eukaryotic cilia. Our data also provide new insights into certain cilium-related mammalian diseases.
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