ArticlePlanta2006
An in vivo, luciferase-based, Agrobacterium-infiltration assay system: implications for post-transcriptional gene silencing.
Article in Planta, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 50 citations in OpenAlex.
- Article
- Effects of Air Temperature on Transient Expression of Influenza Hemagglutinin in Nicotiana benthamiana: Analysis of Transgene Transcription and Plant Stress Responses.Biotechnology and bioengineering · 2025Article
- Critical parameters for robustPlant direct · 2024Article
- A NanoLuc-Based Transactivation Assay in Plants.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Effects of different light conditions on transient expression and biomass inOpen life sciences · 2023Article
- Class I TCP transcription factor AtTCP8 modulates key brassinosteroid-responsive genes.Plant physiology · 2022Article
- A versatile nanoluciferase toolkit and optimized in-gel detection method for protein analysis in plants.Molecular breeding : new strategies in plant improvement · 2021Article
- Oral Immunization With a Plant HSP90-SAG1 Fusion Protein Produced in Tobacco Elicits Strong Immune Responses and Reduces Cyst Number and Clinical Signs of Toxoplasmosis in Mice.Frontiers in plant science · 2021Article
- EASI Transformation: An Efficient Transient Expression Method for Analyzing Gene Function inFrontiers in plant science · 2019Article
- Enhancement of Recombinant Protein Production in TransgenicInternational journal of molecular sciences · 2018Article
- Mature Luffa Leaves (Frontiers in plant science · 2017Article
- Environment Control to Improve Recombinant Protein Yields in Plants Based on Agrobacterium-Mediated Transient Gene Expression.Frontiers in bioengineering and biotechnology · 2016Review
- Within leaf variation is the largest source of variation in agroinfiltration of Nicotiana benthamiana.Plant methods · 2015Article
- Nicotiana small RNA sequences support a host genome origin of cucumber mosaic virus satellite RNA.PLoS genetics · 2015Article
- A reversible Renilla luciferase protein complementation assay for rapid identification of protein-protein interactions reveals the existence of an interaction network involved in xyloglucan biosynthesis in the plant Golgi apparatus.Journal of experimental botany · 2015Article
- Immunogenicity of Brugia malayi Abundant Larval Transcript-2, a potential filarial vaccine candidate expressed in tobacco.Plant cell reports · 2014Article
- Role of the Arabidopsis PIN6 auxin transporter in auxin homeostasis and auxin-mediated development.PloS one · 2013Article
- Rapid in vivo analysis of synthetic promoters for plant pathogen phytosensing.BMC biotechnology · 2011Article
- The maize transcription factor KNOTTED1 directly regulates the gibberellin catabolism gene ga2ox1.The Plant cell · 2009Article
- In vivo characterization of plant promoter element interaction using synthetic promoters.Transgenic research · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An in vivo assay system for analyzing transient luciferase expression in tobacco leaves infused with Agrobacterium tumefaciens is described. The system makes use of A. tumefaciens harboring T-DNA vectors containing either an intron-containing firefly (Photinus pyralis) luciferase (EC 1.13.12.7) gene or an intron-containing sea pansy (Renilla reniformis) luciferase (EC 1.13.12.5) gene. Single or mixed Agrobacterium lines were infiltrated into leaf tissues (Nicotiana tabacum or Nicotiana benthamiana) through stomatal openings and leaf disks from infused areas floated on reaction buffers specific to each enzyme. Photons emitted were then measured to determine reporter gene activity. Parameters affecting assay reliability and sensitivity were tested, including: buffer composition; bacterial density; infusion location; reaction kinetics; and environmental factors (light and temperature). The resulting in vivo assay system generates results comparable to those obtained using a commercially available in vitro dual-luciferase(R) reporter gene assay, and reports relative expression levels, as well as induction characteristics, analogous to those obtained using leaf tissue from stably transformed plants harboring the same promoter::gene constructs. Light and temperature were observed to markedly impact transient reporter activities. Co-expression of viral suppressors of post-transcriptional gene silencing (PTGS), HcPro, p19 and AC2, confirms the occurrence of PTGS within infused zones, and provides a convenient mechanism for PTGS analysis. The in vivo transient assay was used to examine the effect on PTGS of factors such as: promoter strength; incubation temperature and double-stranded RNA production. Results from these assays provide insight into the mechanism(s) used by plants to trigger and maintain PTGS.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.