ArticleAnnals of botany2006
Neutral red as a probe for confocal laser scanning microscopy studies of plant roots.
Article in Annals of botany, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 70 citations in OpenAlex.
- Cortex death precedes stele failure in soybean fine roots during drought but does not prevent plant rehydration.Plant physiology · 2026Article
- Rice cycles between drought and well-watered-adapted phenotypes by changing lateral root formation.Annals of botany · 2025Article
- Label-free structural imaging of plant roots and microbes using third-harmonic generation microscopy.Scientific reports · 2025Article
- Rutin Linoleate Triggers Oxidative Stress-Mediated Cytoplasmic Vacuolation in Non-Small Cell Lung Cancer Cells.Life (Basel, Switzerland) · 2024Article
- Polarity-based fluorescence probes: properties and applications.RSC medicinal chemistry · 2021Review
- Direct fluorescence imaging of lignocellulosic and suberized cell walls in roots and stems.AoB PLANTS · 2020Article
- Technique for the identification of osmophores in flowers of herbarium material (TIOFH).Protoplasma · 2019Article
- Tracking the evolution of CNS remyelinating lesion in mice with neutral red dye.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- THROUGH THE LOOKING GLASS: Real-Time Imaging in Brachypodium Roots and Osmotic Stress Analysis.Plants (Basel, Switzerland) · 2019Article
- Identification of sieve elements and companion cell protoplasts by a combination of brightfield and fluorescence microscopy.Applications in plant sciences · 2018Article
- Protein Storage Vacuoles Originate from Remodeled Preexisting Vacuoles inPlant physiology · 2018Article
- Formation of silica aggregates in sorghum root endodermis is predetermined by cell wall architecture and development.Annals of botany · 2017Article
- Article
- Superior Root Hair Formation Confers Root Efficiency in Some, But Not All, Rice Genotypes upon P Deficiency.Frontiers in plant science · 2016Article
- Differential effects of ephemeral colonization by arbuscular mycorrhizal fungi in two Cuscuta species with different ecology.Mycorrhiza · 2015Article
- Adjustment of host cells for accommodation of symbiotic bacteria: vacuole defunctionalization, HOPS suppression, and TIP1g retargeting in Medicago.The Plant cell · 2014Article
- Following vegetative to embryonic cellular changes in leaves of Arabidopsis overexpressing LEAFY COTYLEDON2.Plant physiology · 2013Article
- Article
- Vacuolar organization in the nodule parenchyma is important for the functioning of pea root nodules.Symbiosis (Philadelphia, Pa.) · 2011Article
- Plasma membrane domains participate in pH banding of Chara internodal cells.Plant & cell physiology · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsNeutral red (NR), a lipophilic phenazine dye, has been widely used in various biological systems as a vital stain for bright-field microscopy. In its unprotonated form it penetrates the plasma membrane and tonoplast of viable plant cells, then due to protonation it becomes trapped in acidic compartments. The possible applications of NR for confocal laser scanning microscopy (CLSM) studies were examined in various aspects of plant root biology.
methodsNR was used as a fluorochrome for living roots of Phaseolus vulgaris, Allium cepa, A. porrum and Arabidopsis thaliana (wild-type and transgenic GFP-carrying lines). The tissues were visualized using CLSM. The effect of NR on the integrity of the cytoskeleton and the growth rate of arabidopsis primary roots was analysed to judge potential toxic effects of the dye. KEY
resultsThe main advantages of the use of NR are related to the fact that NR rapidly penetrates root tissues, has affinity to suberin and lignin, and accumulates in the vacuoles. It is shown that NR is a suitable probe for visualization of proto- and metaxylem elements, Casparian bands in the endodermis, and vacuoles in cells of living roots. The actin cytoskeleton and the microtubule system of the cells, as well as the dynamics of root growth, remain unchanged after short-term application of NR, indicating a relatively low toxicity of this chemical. It was also found that NR is a useful probe for the observation of the internal structures of root nodules and of fungal hyphae in vesicular-arbuscular mycorrhizas.
conclusionsEase, low cost and absence of tissue processing make NR a useful probe for structural, developmental and vacuole-biogenetic studies of plant roots with CLSM.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.