ArticleThe Journal of cell biology1991
Modulation of contraction by gelation/solation in a reconstituted motile model.
Article in The Journal of cell biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
59 citing papers in PubMed, 112 citations in OpenAlex.
- Reconstitution of actomyosin networks in cell-sized liposomes dissects distinct mechanisms of membrane blebbing and symmetry breaking.Science advances · 2026Article
- Reconstitution of actomyosin networks in cell-sized liposomes reveals distinct mechanical roles of cytoskeletal organization in membrane shape remodeling.bioRxiv : the preprint server for biology · 2025Article
- Mechanics of cell sheets: plectin as an integrator of cytoskeletal networks.Open biology · 2025Review
- Myosin-induced F-actin fragmentation facilitates contraction of actin networks.Cytoskeleton (Hoboken, N.J.) · 2024Article
- Actomyosin Contraction Induces In-Bulk Motility of Cells and Droplets.Biophysical journal · 2020Article
- The role of the Arp2/3 complex in shaping the dynamics and structures of branched actomyosin networks.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- A High-Throughput Workflow to Study Remodeling of Extracellular Matrix-Based Microtissues.Tissue engineering. Part C, Methods · 2019Article
- Oscillatory fluid flow drives scaling of contraction wave with system size.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Emergent mechanics of actomyosin drive punctuated contractions and shape network morphology in the cell cortex.PLoS computational biology · 2018Article
- Filament turnover tunes both force generation and dissipation to control long-range flows in a model actomyosin cortex.PLoS computational biology · 2017Article
- Filament rigidity and connectivity tune the deformation modes of active biopolymer networks.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Article
- Plastin increases cortical connectivity to facilitate robust polarization and timely cytokinesis.The Journal of cell biology · 2017Article
- Architecture and Connectivity Govern Actin Network Contractility.Current biology : CB · 2016Article
- Forcing cells into shape: the mechanics of actomyosin contractility.Nature reviews. Molecular cell biology · 2015Review
- Actomyosin sliding is attenuated in contractile biomimetic cortices.Molecular biology of the cell · 2014Article
- Reconstitution of contractile actomyosin arrays.Methods in enzymology · 2014Article
- The antagonistic modulation of Arp2/3 activity by N-WASP, WAVE2 and PICK1 defines dynamic changes in astrocyte morphology.Journal of cell science · 2013Article
- Molecular characterization of severin from Clonorchis sinensis excretory/secretory products and its potential anti-apoptotic role in hepatocarcinoma PLC cells.PLoS neglected tropical diseases · 2013Article
- Mathematical modeling of eukaryotic cell migration: insights beyond experiments.Annual review of cell and developmental biology · 2013Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The actin-based cytoskeleton is a dynamic component of living cells with major structural and contractile properties involved in fundamental cellular processes. The action of actin-binding proteins can decrease or increase the gel structure. Changes in the actin-based cytoskeleton have long been thought to modulate the myosin II-based contractions involved in these cellular processes, but there has been some debate concerning whether maximal gelation increases or decreases contractile activity. To address this question, we have examined how contractile activity is modulated by the extent of actin gelation. The model system consists of physiologically relevant concentrations and molar ratios of actin filaments (whose lengths are controlled by gelsolin), the actin-cross-linking protein filamin, and smooth muscle myosin II. This system has been studied at the macroscopic and light microscopic levels to relate the gel structure to the rate of contraction. We present results which show that while a minimal amount of structure is necessary to transmit the contractile force, increasing the gel structure inhibits the rate of contraction, despite an increase in the actin-activated Mg(2+)-ATPase activity of myosin. Decreasing the total myosin concentration also inhibits the rate of contraction. Application of cytochalasin D to one side of the contractile network increases the rate of contraction and also induces movement comparable to flare streaming observed in isolated amoeba cytoplasm. These results are interpreted relative to current models of the relationship between the state of gelation and contraction and to the potential effects of such a relationship in the living cell.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.