ArticlePlant cell reports2006
A high throughput Agrobacterium tumefaciens-mediated transformation method for functional genomics of perennial ryegrass (Lolium perenne L.).
Article in Plant cell reports, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 53 citations in OpenAlex.
- Genetic transformation of forage crops: comparative barriers, evidence, and emerging strategies.Plant cell reports · 2026Review
- Callus Induction from Diverse Explants and Genotypes Enables Robust Transformation of Perennial Ryegrass (Plants (Basel, Switzerland) · 2022Article
- Changes in Leaf-Level Nitrogen Partitioning and Mesophyll Conductance Deliver Increased Photosynthesis forFrontiers in plant science · 2021Article
- Isopentenyl Transferase (IPT) Gene Transfer to Perennial Ryegrass Through Sonication-Assisted Agrobacterium-Mediated Transformation (SAAT), Vacuum and Heat Treatment.Molecular biotechnology · 2019Article
- Improving the Yield and Nutritional Quality of Forage Crops.Frontiers in plant science · 2018Review
- Overexpression of a Chimeric Gene, OsDST-SRDX, Improved Salt Tolerance of Perennial Ryegrass.Scientific reports · 2016Article
- Advances in Agrobacterium tumefaciens-mediated genetic transformation of graminaceous crops.Protoplasma · 2016Review
- Reduced lignin content and altered lignin composition in the warm season forage grass Paspalum dilatatum by down-regulation of a Cinnamoyl CoA reductase gene.Transgenic research · 2014Article
- Enhanced Agrobacterium-mediated transformation efficiencies in monocot cells is associated with attenuated defense responses.Plant molecular biology · 2013Article
- Brachypodium sylvaticum, a model for perennial grasses: transformation and inbred line development.PloS one · 2013Article
- Validation of reference genes for quantitative RT-PCR studies of gene expression in perennial ryegrass (Lolium perenne L.).BMC molecular biology · 2010Article
- Comparative analysis of transgenic tall fescue (Festuca arundinacea Schreb.) plants obtained by Agrobacterium-mediated transformation and particle bombardment.Plant cell reports · 2008Article
- A high-throughput Agrobacterium-mediated transformation system for the grass model species Brachypodium distachyon L.Transgenic research · 2008Article
- Agrobacterium tumefaciens-mediated transformation of Narcissus tazzeta var. chinensis.Plant cell reports · 2007Article
- Generation of transgenic Lolium temulentum plants by Agrobacterium tumefaciens-mediated transformation.Plant cell reports · 2007Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A robust and high throughput Agrobacterium genetic transformation procedure has been developed for perennial ryegrass (Lolium perenne L.). Embryogenic callus lines were selected and maintained as plants in vitro. Embryogenic calli derived from meristematic regions of the vegetative tillers were co-cultivated with Agrobacterium tumefaciens strain EHA101 carrying the plasmid pCAMBIA 1305.1 in the presence of acetosyringone for 3-4 days. The calli were grown under 94.8 and 151.6 microM hygromycin selection, respectively for two cycles of 2-weeks each, followed by transfer to regeneration medium with 47.4 microM hygromycin. Regenerated plants were rooted and successfully transferred to soil. The transgenic nature of the regenerated plants was confirmed by DNA gel blot analysis and gene expression demonstrated by GUS histochemical assay and/or reverse transcription PCR. After development of the transformation procedure, we used Agrobacterium strain EHA101 carrying a modified binary plasmid pMH bearing genes of interest. In the past 2 years, we have produced more than 1,000 plants with constructs encoding different genes of interest from perennial ryegrass.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.