ArticlePlant cell reports2006
Simplified and improved somatic embryogenesis for clonal propagation of Pinus pinaster (Ait.).
Article in Plant cell reports, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Somatic embryogenesis of Arabica coffee in temporary immersion culture: Advances, limitations, and perspectives for mass propagation of selected genotypes.Frontiers in plant science · 2022Pooled it
- An improved and simplified somatic embryogenesis protocol in Chir pine (Pinus roxburghii).Scientific reports · 2025Article
- Transcriptome Analysis of Embryogenic and Non-Embryogenic Callus ofCurrent issues in molecular biology · 2023Article
- Application of Cold Storage and Short In Vitro Germination for Somatic Embryos ofPlants (Basel, Switzerland) · 2023Article
- Reducing Pre- and Post-Treatments in Cryopreservation Protocol and Testing Storage at -80 °C for Norway Spruce Embryogenic Cultures.International journal of molecular sciences · 2022Article
- Somatic Embryogenesis in Pines.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Norway spruce somatic embryogenesis benefits from proliferation of embryogenic tissues on filter discs and cold storage of cotyledonary embryos.Frontiers in plant science · 2022Article
- Somatic Embryogenesis and Plant Regeneration from Sugi (Japanese Cedar,Plants (Basel, Switzerland) · 2020Article
- New Approaches to Optimize Somatic Embryogenesis in Maritime Pine.Frontiers in plant science · 2019Article
- Progress in Somatic Embryogenesis of Japanese Pines.Frontiers in plant science · 2019Review
- Improved and synchronized maturation of Norway spruce (In vitro cellular & developmental biology. Plant : journal of the Tissue Culture Association · 2018Article
- Carbohydrate-mediated responses during zygotic and early somatic embryogenesis in the endangered conifer, Araucaria angustifolia.PloS one · 2017Article
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- Stable Agrobacterium-mediated transformation of maritime pine based on kanamycin selection.TheScientificWorldJournal · 2013Article
- Analysis of genetic stability at SSR loci during somatic embryogenesis in maritime pine (Pinus pinaster).Plant cell reports · 2009Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, several improvements and simplifications of SE protocols in Pinus pinaster (Ait.), a species of economic importance in the regions of Western Europe, are described. These improvements pertained to all stages of SE including high initiation frequencies in eight control pollinated seed families, relatively high somatic embryo maturation yield when cells were coated with particles of activated charcoal and a rapid production of plants directly in a shade house. The SE initiation frequency from isolated zygotic embryos was high (up to 100%) and plants were produced from 11 embryogenic lines representing all crosses. Based on these results, the estimated number of somatic embryos required to produce 1,000 plants varied from slightly more than the required number of plants to more than double this number depending on the line. Such an estimate is critical in developing plant production strategy when a number of embryogenic lines are considered for production of clonal plants.
Indexed as
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16518635What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.