ArticleNucleic acids research2006
Homogeneous point mutation detection by quantum dot-mediated two-color fluorescence coincidence analysis.
Article in Nucleic acids research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Advances in nucleic acid probe-based detection of gene point mutations: a review.Frontiers in chemistry · 2025Review
- Recent Advances in Single-Molecule Tracking and Imaging Techniques.Annual review of analytical chemistry (Palo Alto, Calif.) · 2023Review
- Digital Receptor Occupancy Assay in Quantifying On- and Off-Target Binding Affinities of Therapeutic Antibodies.ACS sensors · 2020Article
- Article
- A flow-proteometric platform for analyzing protein concentration (FAP): Proof of concept for quantification of PD-L1 protein in cells and tissues.Biosensors & bioelectronics · 2018Article
- LNA Thymidine Monomer Enables Differentiation of the Four Single-Nucleotide Variants by Melting Temperature.Journal of the American Chemical Society · 2017Article
- Magnetic ligation method for quantitative detection of microRNAs.Advanced healthcare materials · 2014Article
- Detection, counting, and imaging of single nanoparticles.Analytical chemistry · 2014Review
- Single quantum dot analysis enables multiplexed point mutation detection by gap ligase chain reaction.Small (Weinheim an der Bergstrasse, Germany) · 2013Article
- A fluorescence light-up Ag nanocluster probe that discriminates single-nucleotide variants by emission color.Journal of the American Chemical Society · 2012Article
- Quantum dot enabled molecular sensing and diagnostics.Theranostics · 2012Article
- Decoding circulating nucleic acids in human serum using microfluidic single molecule spectroscopy.Journal of the American Chemical Society · 2010Article
- Counting single molecules in sub-nanolitre droplets.Lab on a chip · 2010Article
- Microfluidic means of achieving attomolar detection limits with molecular beacon probes.Lab on a chip · 2009Article
- Polymer nanofibre junctions of attolitre volume serve as zeptomole-scale chemical reactors.Nature chemistry · 2009Article
- Tunable blinking kinetics of cy5 for precise DNA quantification and single-nucleotide difference detection.Biophysical journal · 2008Article
- Potential clinical applications of quantum dots.International journal of nanomedicine · 2008Review
- Quantification of low concentrations of DNA using single molecule detection and velocity measurement in a microchannel.Journal of fluorescence · 2007Article
- A microfluidic-FCS platform for investigation on the dissociation of Sp1-DNA complex by doxorubicin.Nucleic acids research · 2006Article
- Fluorescent Detection of Methicillin ResistantAvicenna journal of medical biotechnologyArticle
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This report describes a new genotyping method capable of detecting low-abundant point mutations in a homogeneous, separation-free format. The method is based on integration of oligonucleotide ligation with a semiconductor quantum dot (QD)-mediated two-color fluorescence coincidence detection scheme. Surface-functionalized QDs are used to capture fluorophore-labeled ligation products, forming QD-oligonucleotide nanoassemblies. The presence of such nanoassemblies and thereby the genotype of the sample is determined by detecting the simultaneous emissions of QDs and fluorophores that occurs whenever a single nanoassembly flows through the femtoliter measurement volume of a confocal fluorescence detection system. The ability of this method to detect single events enables analysis of target signals with a multiple-parameter (intensities and count rates of the digitized target signals) approach to enhance assay sensitivity and specificity. We demonstrate that this new method is capable of detecting zeptomoles of targets and achieve an allele discrimination selectivity factor >10(5).
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.