ArticleJournal of clinical microbiology2006
Molecular subtyping of poultry-associated type A Clostridium perfringens isolates by repetitive-element PCR.
Article in Journal of clinical microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Evaluation of the efficacy of chlorogenic acid in reducing small intestine injury, oxidative stress, and inflammation in chickens challenged with Clostridium perfringens type A.Poultry science · 2020Article
- Prevalence and multilocus sequence typing of Clostridium perfringens isolated from 4 duck farms in Shandong province, China.Poultry science · 2020Article
- Molecular detection and characterization of Clostridium perfringens toxin genes causing necrotic enteritis in broiler chickens.Tropical animal health and production · 2019Article
- Genomic diversity ofIranian journal of veterinary research · 2016Article
- Article
- Expression of a Clostridium perfringens genome-encoded putative N-acetylmuramoyl-L-alanine amidase as a potential antimicrobial to control the bacterium.Archives of microbiology · 2013Article
- Article
- Comparative genomics of four closely related Clostridium perfringens bacteriophages reveals variable evolution among core genes with therapeutic potential.BMC genomics · 2011Article
- Multilocus sequence typing subtypes of poultry Clostridium perfringens isolates demonstrate disease niche partitioning.Journal of clinical microbiology · 2011Article
- Clostridium perfringens bacteriophages ΦCP39O and ΦCP26F: genomic organization and proteomic analysis of the virions.Archives of virology · 2011Article
- Recombinant expression of two bacteriophage proteins that lyse clostridium perfringens and share identical sequences in the C-terminal cell wall binding domain of the molecules but are dissimilar in their N-terminal active domains.Journal of agricultural and food chemistry · 2010Article
- Comparison and utilization of repetitive-element PCR techniques for typing Lactobacillus isolates from the chicken gastrointestinal tract.Applied and environmental microbiology · 2009Article
- The rapid identification of Clostridium perfringens as the possible aetiology of a diarrhoeal outbreak using PCR.Epidemiology and infection · 2008Article
- Role of law enforcement response and microbial forensics in investigation of bioterrorism.Croatian medical journal · 2007Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clostridium perfringens strains (type A) isolated from an integrated poultry operation were subtyped using repetitive-element PCR with Dt primers. Isolates were obtained from fecal, egg shell, fluff, and carcass rinse samples as part of a previously reported temporally linked epidemiological survey. A total of 48 isolates of C. perfringens were obtained from different stages of the broiler chicken production chain from two separate breeder farms that supplied a single hatchery that in turn provided chicks to a single grow-out farm whose flocks were processed at a single plant. All 48 isolates were typeable (100% typeability) by repetitive-element PCR with Dt primers. This subtyping method was highly reproducible and discriminatory. By repetitive-element PCR with Dt primers, isolates were classified into four major branches with 12 subgroups or clades. The Simpson's index of discrimination was calculated to be 0.96 for groupings of >95% correlation. Toxin gene profiles of the isolates indicated that all of the isolates were C. perfringens alpha-toxin gene positive and 46 of 48 isolates were beta2-toxin gene positive. All strains were negative for beta- and epsilon-toxin genes. Repetitive sequence-based PCR was found to be a technically practical and reproducible means of subtyping C. perfringens libraries from specific epidemiological or production environment settings.
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