Evidence map›Paper›PMID 16517880›Full record

ArticleJournal of clinical microbiology2006

Comparison of culture and a novel 5' Taq nuclease assay for direct detection of Campylobacter fetus subsp. venerealis in clinical specimens from cattle.

Lyle McMillen, Geoffry Fordyce, Vivienne J Doogan, Ala E Lew

Abstract readComparative StudyEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of clinical microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Bovine campylobacteriosis in bulls: insights in the conventional and molecular diagnosis.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023
    Article
  6. Article
  7. Article
  8. Not gone but forgotten:Current research in parasitology & vector-borne diseases · 2021
    Article
  9. Article
  10. Article
  11. Genome Sequence of Campylobacter fetus subsp.Microbiology resource announcements · 2020
    Article
  12. Phylogenomic Analysis ofFrontiers in microbiology · 2020
    Article
  13. Review
  14. Evaluation of long-acting oxytetracycline and a commercial monovalent vaccine for the control ofThe Canadian veterinary journal = La revue veterinaire canadienne · 2017
    Article
  15. Moving past serology: Diagnostic options without serum.Veterinary journal (London, England : 1997) · 2016
    Review
  16. Article
  17. Article
  18. Article
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Lyle McMillenQueensland Department of Primary Industries and Fisheries, c/o Animal Research Institute, Locked Mail Bag No. 4, Moorooka, 4105 QLD, Australia. ala.lew@dpi.qld.gov.au.
Geoffry Fordyce
Vivienne J Doogan
Ala E Lew

Funding

Lnc-PINK regulation of innate immunity in lung epithelial cellsR21AI152004 · NIAID · OKLAHOMA STATE UNIVERSITY STILLWATER · PI LIU, LIN · 2021 to 2022
$411k
NIAID NIH HHS R21 AI152004
6 · The paper itself

Abstract

A Campylobacter fetus subsp. venerealis-specific 5' Taq nuclease PCR assay using a 3' minor groove binder-DNA probe (TaqMan MGB) was developed based on a subspecies-specific fragment of unknown identity (S. Hum, K. Quinn, J. Brunner, and S. L. On, Aust. Vet. J. 75:827-831, 1997). The assay specifically detected four C. fetus subsp. venerealis strains with no observed cross-reaction with C. fetus subsp. fetus-related Campylobacter species or other bovine venereal microflora. The 5' Taq nuclease assay detected approximately one single cell compared to 100 and 10 cells in the conventional PCR assay and 2,500 and 25,000 cells from selective culture from inoculated smegma and mucus, respectively. The respective detection limits following the enrichments from smegma and mucus were 5,000 and 50 cells/inoculum for the conventional PCR compared to 500 and 50 cells/inoculum for the 5' Taq nuclease assay. Field sampling confirmed the sensitivity and the specificity of the 5' Taq nuclease assay by detecting an additional 40 bulls that were not detected by culture. Urine-inoculated samples demonstrated comparable detection of C. fetus subsp. venerealis by both culture and the 5' Taq nuclease assay; however, urine was found to be less effective than smegma for bull sampling. Three infected bulls were tested repetitively to compare sampling tools, and the bull rasper proved to be the most suitable, as evidenced by the improved ease of specimen collection and the consistent detection of higher levels of C. fetus subsp. venerealis. The 5' Taq nuclease assay demonstrates a statistically significant association with culture (chi2 = 29.8; P < 0.001) and significant improvements for the detection of C. fetus subsp. venerealis-infected animals from crude clinical extracts following prolonged transport.

Indexed as

AnimalsBacteriological TechniquesBase SequenceCampylobacter fetusCampylobacter InfectionsCattleCattle DiseasesDNA, BacterialDNA Restriction EnzymesFemaleMalePolymerase Chain ReactionSensitivity and SpecificityDNA, BacterialDNA Restriction Enzymes

Identifiers

PMID16517880
PMCPMC1393111

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.