ArticleJournal of clinical microbiology2006
Simultaneous detection and identification of Candida, Aspergillus, and Cryptococcus species by reverse line blot hybridization.
Article in Journal of clinical microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Nucleic Acid Amplification Tests forPathogens (Basel, Switzerland) · 2026Pooled it
- Small Molecular Contaminant and Microorganism Can Be Simultaneously Detected Based on Nanobody-Phage: Using Carcinogen Aflatoxin and Its Main FungalFrontiers in microbiology · 2019Article
- Article
- Practical identification of eight medically important Trichosporon species by reverse line blot hybridization (RLB) assay and rolling circle amplification (RCA).Medical mycology · 2013Article
- Simultaneous detection and identification of Aspergillus and mucorales species in tissues collected from patients with fungal rhinosinusitis.Journal of clinical microbiology · 2011Article
- High-throughput identification and quantification of Candida species using high resolution derivative melt analysis of panfungal amplicons.The Journal of molecular diagnostics : JMD · 2010Article
- Rapid detection of ERG11 gene mutations in clinical Candida albicans isolates with reduced susceptibility to fluconazole by rolling circle amplification and DNA sequencing.BMC microbiology · 2009Article
- Development and validation of a microsphere-based Luminex assay for rapid identification of clinically relevant aspergilli.Journal of clinical microbiology · 2009Article
- Surface plasmon resonance detection using antibody-linked magnetic nanoparticles for analyte capture, purification, concentration, and signal amplification.Analytical chemistry · 2009Article
- Detection of a molecular biomarker for zygomycetes by quantitative PCR assays of plasma, bronchoalveolar lavage, and lung tissue in a rabbit model of experimental pulmonary zygomycosis.Journal of clinical microbiology · 2008Article
- Evaluation of novel broad-range real-time PCR assay for rapid detection of human pathogenic fungi in various clinical specimens.Journal of clinical microbiology · 2008Article
- Rapid identification and differentiation of Trichophyton species, based on sequence polymorphisms of the ribosomal internal transcribed spacer regions, by rolling-circle amplification.Journal of clinical microbiology · 2008Article
- New method for simultaneous species-specific identification of equine strongyles (nematoda, strongylida) by reverse line blot hybridization.Journal of clinical microbiology · 2007Article
- Reverse line blot hybridization assay for identification of medically important fungi from culture and clinical specimens.Journal of clinical microbiology · 2007Article
- Identification of medically important Candida and non-Candida yeast species by an oligonucleotide array.Journal of clinical microbiology · 2007Article
- Development and clinical application of a panfungal PCR assay to detect and identify fungal DNA in tissue specimens.Journal of clinical microbiology · 2007Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report on a reverse line blot (RLB) assay, utilizing fungal species-specific oligonucleotide probes to hybridize with internal transcribed spacer 2 region sequences amplified using a nested panfungal PCR. Reference and clinical strains of 16 Candida species (116 strains), Cryptococcus neoformans (five strains of Cryptococcus neoformans var. neoformans, five strains of Cryptococcus neoformans var. grubii, and six strains of Cryptococcus gatti), and five Aspergillus species (68 strains) were all correctly identified by the RLB assay. Additional fungal species (16 species and 26 strains) not represented on the assay did not exhibit cross-hybridization with the oligonucleotide probes. In simulated clinical specimens, the sensitivity of the assay for Candida spp. and Aspergillus spp. was 10(0.5) cells/ml and 10(2) conidia/ml, respectively. This assay allows sensitive and specific simultaneous detection and identification of a broad range of fungal pathogens.
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