ArticleJournal of clinical microbiology2006
Identification of medically important yeast species by sequence analysis of the internal transcribed spacer regions.
Article in Journal of clinical microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 132 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
132 citing papers in PubMed, 311 citations in OpenAlex.
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- Prevalence of dermatophytosis in cats and dogs in türkiye: dominance of Microsporum canis and first detection of Trichophyton rubrum.BMC veterinary research · 2025Article
- Screening of yeasts in the oral microbiota of bats (Chiroptera) in Piauí, Brazil.New microbes and new infections · 2025Article
- Environmental Yeast Abundance and Diversity Assessment in Recreation Areas of Bangkok, Thailand.Environmental microbiology reports · 2025Article
- Expansion of the Candidozyma haemuli Species Complex - The Novel Species Candidozyma molenica, Isolated from Clinical and Environmental Sources.Mycopathologia · 2025Article
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- Characterization of Zalaria obscura Y1223 hydrolases: implications for lignocellulose conversion.AMB Express · 2025Article
- Genome sequence of the yeastMicrobiology resource announcements · 2025Article
- Genome sequence of the yeastMicrobiology resource announcements · 2025Article
- Characterization of Red Dragon Fruit Wine Fermented with a Newly Identified Yeast StrainFood technology and biotechnology · 2025Article
- Lactose-assimilating yeasts with high fatty acid accumulation uncovered by untargeted bioprospecting.Applied and environmental microbiology · 2025Article
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- Xylitol promotes the antioxidant and biocontrol efficiency of the antagonistic yeast,Frontiers in microbiology · 2025Article
- ICU care through NGS - Based identification of infectious agents: A comparative study.Heliyon · 2024Article
- PCR-Based Microarray Enhances Diagnosis of Culture-Negative Biopsied Tissue in Patients with Invasive Mold Infections: Real-World Experience in a Tertiary Medical Center.Journal of fungi (Basel, Switzerland) · 2024Article
- First Data on the Investigation of Gut Yeasts in Hermit Beetle (Journal of fungi (Basel, Switzerland) · 2024Article
- Exploring the diversity of uncommon oral yeast species and associated risk factors among substance abusers in southwestern Iran.Scientific reports · 2024Article
72 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Infections caused by yeasts have increased in previous decades due primarily to the increasing population of immunocompromised patients. In addition, infections caused by less common species such as Pichia, Rhodotorula, Trichosporon, and Saccharomyces spp. have been widely reported. This study extensively evaluated the feasibility of sequence analysis of the rRNA gene internal transcribed spacer (ITS) regions for the identification of yeasts of clinical relevance. Both the ITS1 and ITS2 regions of 373 strains (86 species), including 299 reference strains and 74 clinical isolates, were amplified by PCR and sequenced. The sequences were compared to reference data available at the GenBank database by using BLAST (basic local alignment search tool) to determine if species identification was possible by ITS sequencing. Since the GenBank database currently lacks ITS sequence entries for some yeasts, the ITS sequences of type (or reference) strains of 15 species were submitted to GenBank to facilitate identification of these species. Strains producing discrepant identifications between the conventional methods and ITS sequence analysis were further analyzed by sequencing of the D1-D2 domain of the large-subunit rRNA gene for species clarification. The rates of correct identification by ITS1 and ITS2 sequence analysis were 96.8% (361/373) and 99.7% (372/373), respectively. Of the 373 strains tested, only 1 strain (Rhodotorula glutinis BCRC 20576) could not be identified by ITS2 sequence analysis. In conclusion, identification of medically important yeasts by ITS sequencing, especially using the ITS2 region, is reliable and can be used as an accurate alternative to conventional identification methods.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.