ArticleApplied and environmental microbiology2006
Evaluation of 23S rRNA PCR primers for use in phylogenetic studies of bacterial diversity.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
48 citing papers in PubMed.
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- Superior Resolution Profiling of the Coleofasciculus Microbiome by Amplicon Sequencing of the Complete 16S rRNA Gene and ITS Region.Environmental microbiology reports · 2025Article
- PrimerEvalPy: a tool for in-silico evaluation of primers for targeting the microbiome.BMC bioinformatics · 2024Article
- Article
- Ultrastructural and proteomic evidence for the presence of a putative nucleolus in an Archaeon.Frontiers in microbiology · 2023Article
- Standardized multi-omics of Earth's microbiomes reveals microbial and metabolite diversity.Nature microbiology · 2022Article
- High-resolution phylogenetic and population genetic analysis of microbial communities with RoC-ITS.ISME communications · 2022Article
- Sugarcane Genotypes with Contrasting Biological Nitrogen Fixation Efficiencies Differentially Modulate Nitrogen Metabolism, Auxin Signaling, and Microorganism Perception Pathways.Plants (Basel, Switzerland) · 2022Article
- Histopathological Findings and Metagenomic Analysis of Esophageal Papillary Proliferation Identified in Laying Broiler Breeders.Veterinary sciences · 2022Article
- Development of spirulina for the manufacture and oral delivery of protein therapeutics.Nature biotechnology · 2022Article
- Fatal Infection in an Alpaca (Animals : an open access journal from MDPI · 2022Article
- Sequence-Specific Capture of Oligonucleotide Probes (SCOPE): a Simple and Rapid Microbial rRNA Quantification Method Using a Molecular Weight Cutoff Membrane.Applied and environmental microbiology · 2021Article
- Early-life effects of juvenile Western diet and exercise on adult gut microbiome composition in mice.The Journal of experimental biology · 2021Article
- High-accuracy long-read amplicon sequences using unique molecular identifiers with Nanopore or PacBio sequencing.Nature methods · 2021Article
- Species-level evaluation of the human respiratory microbiome.GigaScience · 2020Article
- High Levels of Oxidative Stress Create a Microenvironment That Significantly Decreases the Diversity of the Microbiota in Diabetic Chronic Wounds and Promotes Biofilm Formation.Frontiers in cellular and infection microbiology · 2020Article
- High Levels of Oxidative Stress and Skin Microbiome are Critical for Initiation and Development of Chronic Wounds in Diabetic Mice.Scientific reports · 2019Article
- Diverse, Abundant, and Novel Viruses Infecting the MarinemSystems · 2019Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The availability of a diverse set of 23S rRNA gene sequences enabled evaluation of the specificity of 39 previously published and 4 newly designed primers specific for bacteria. An extensive clone library constructed using an optimized primer pair resulted in similar gene richness but slightly differing coverage of some phylogenetic groups, compared to a 16S rRNA gene library from the same environmental sample.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.