ArticleApplied and environmental microbiology2006
Metabolic engineering of Corynebacterium glutamicum for trehalose overproduction: role of the TreYZ trehalose biosynthetic pathway.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 52 citations in OpenAlex.
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- Production of the compatible solute α-D-glucosylglycerol by metabolically engineered Corynebacterium glutamicum.Microbial cell factories · 2018Article
- Rapid, label-free CD4 testing using a smartphone compatible device.Lab on a chip · 2017Article
- Engineering long shelf life multi-layer biologically active surfaces on microfluidic devices for point of care applications.Scientific reports · 2016Article
- Biocatalytic Production of Trehalose from Maltose by Using Whole Cells of Permeabilized Recombinant Escherichia coli.PloS one · 2015Article
- Trends in bacterial trehalose metabolism and significant nodes of metabolic pathway in the direction of trehalose accumulation.Microbial biotechnology · 2013Review
- Development and application of an arabinose-inducible expression system by facilitating inducer uptake in Corynebacterium glutamicum.Applied and environmental microbiology · 2012Article
- Improved trehalose production from biodiesel waste using parent and osmotically sensitive mutant of Propionibacterium freudenreichii subsp. shermanii under aerobic conditions.Journal of industrial microbiology & biotechnology · 2012Article
- Effect of trehalose on protein structure.Protein science : a publication of the Protein Society · 2009Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Trehalose has many potential applications in biotechnology and the food industry due to its protective effect against environmental stress. Our work explores microbiological production methods based on the capacity of Corynebacterium glutamicum to excrete trehalose. We address here raising trehalose productivity through homologous overexpression of maltooligosyltrehalose synthase and the maltooligosyltrehalose trehalohydrolase genes. In addition, heterologous expression of the UDP-glucose pyrophosphorylase gene from Escherichia coli improved the supply of glycogen. Gene expression effects were tested on enzymatic activities and intracellular glycogen content, as well as on accumulated and excreted trehalose. Overexpression of the treY gene and the treY/treZ synthetic operon significantly increased maltooligosyltrehalose synthase activity, the rate-limiting step, and improved the specific productivity and the final titer of trehalose. Furthermore, a strong decrease was noted in glycogen accumulation. Expression of galU/treY and galU/treYZ synthetic operons showed a partial recovery in the intracellular glycogen levels and a significant improvement in both intra- and extracellular trehalose content.
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