ArticleApplied and environmental microbiology2006
Freeze-thaw tolerance and clues to the winter survival of a soil community.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Winter and early spring COScientific reports · 2026Article
- Antarctic microbial protein-rich extracts with cryoprotective potential for cell and viral preservation.Frontiers in microbiology · 2026Article
- From Microbial Consortia to Ecosystem Resilience: The Integrative Roles of Holobionts in Stress Biology.Biology · 2025Article
- Study on the effects of winter irrigation during seasonal freezing-thawing period on soil microbial ecological properties.Scientific reports · 2025Article
- Accelerating Gut Microbiome Research with Robust Sample Collection.Research & reviews. Journal of microbiology and biotechnology · 2023Article
- NEcology and evolution · 2023Article
- Mining Biosynthetic Gene Clusters inMicroorganisms · 2022Article
- Antibiotic resistance in mucosal bacteria from high Arctic migratory salmonids.Environmental microbiology reports · 2022Article
- Water is a preservative of microbes.Microbial biotechnology · 2022Article
- Effects of autumn diurnal freeze-thaw cycles on soil bacteria and greenhouse gases in the permafrost regions.Frontiers in microbiology · 2022Article
- Responses of soil bacterial communities to precipitation change in the semi-arid alpine grassland of Northern Tibet.Frontiers in plant science · 2022Article
- Root-Associated Mycobiomes of Common Temperate Plants (Calluna vulgaris and Holcus lanatus) Are Strongly Affected by Winter Climate Conditions.Microbial ecology · 2021Article
- Diversity and Antimicrobial Activity towardsFoods (Basel, Switzerland) · 2021Article
- Cryopreservation of clonal and polyclonal populations ofBiology methods & protocols · 2021Article
- Impacts of different freeze-thaw treatments on the adsorption and desorption behaviors of Cd in black soil.Environmental science and pollution research international · 2020Article
- Soil microbial community responses to climate extremes: resistance, resilience and transitions to alternative states.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Article
- Functional and Structural Responses of Arctic and Alpine Soil Prokaryotic and Fungal Communities Under Freeze-Thaw Cycles of Different Frequencies.Frontiers in microbiology · 2020Article
- Total and Potentially Active Bacterial Communities Entrapped in a Late Glacial Through Holocene Ice Core From Scarisoara Ice Cave, Romania.Frontiers in microbiology · 2019Article
- Natural freeze-thaw cycles may increase the risk associated withWater research X · 2018Article
- Contrasting Soil Bacterial Community, Diversity, and Function in Two Forests in China.Frontiers in microbiology · 2018Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although efforts have been made to sample microorganisms from polar regions and to investigate a few of the properties that facilitate survival at freezing or subzero temperatures, soil communities that overwinter in areas exposed to alternate freezing and thawing caused by Foehn or Chinook winds have been largely overlooked. We designed and constructed a cryocycler to automatically subject soil cultures to alternating freeze-thaw cycles. After 48 freeze-thaw cycles, control Escherichia coli and Pseudomonas chlororaphis isolates were no longer viable. Mixed cultures derived from soil samples collected from a Chinook zone showed that the population complexity and viability were reduced after 48 cycles. However, when bacteria that were still viable after the freeze-thaw treatments were used to obtain selected cultures, these cultures proved to be >1,000-fold more freeze-thaw tolerant than the original consortium. Single-colony isolates obtained from survivors after an additional 48 freeze-thaw cycles were putatively identified by 16S RNA gene fragment sequencing. Five different genera were recognized, and one of the cultures, Chryseobacterium sp. strain C14, inhibited ice recrystallization, a property characteristic of antifreeze proteins that prevents the growth of large, potentially damaging ice crystals at temperatures close to the melting temperature. This strain was also notable since cell-free medium derived from cultures of it appeared to enhance the multiple freeze-thaw survival of another isolate, Enterococcus sp. strain C8. The results of this study and the development of a cryocycler should allow further investigations into the biochemical and soil community adaptations to the rigors of a Chinook environment.
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