ArticleProceedings of the National Academy of Sciences of the United States of America1991
Amiloride-sensitive sodium channel is linked to the cytoskeleton in renal epithelial cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 188 citations in OpenAlex.
- Epithelial NaComprehensive Physiology · 2024Review
- Pleiotropic Ankyrins: Scaffolds for Ion Channels and Transporters.Channels (Austin, Tex.) · 2022Review
- Ankyrin G Expression Regulates Apical Delivery of the Epithelial Sodium Channel (ENaC).The Journal of biological chemistry · 2017Article
- The Epithelial Sodium Channel and the Processes of Wound Healing.BioMed research international · 2016Review
- Cytochalasin E alters the cytoskeleton and decreases ENaC activity in Xenopus 2F3 cells.American journal of physiology. Renal physiology · 2014Article
- The role of ENaC in vascular endothelium.Pflugers Archiv : European journal of physiology · 2014Review
- Blood pressure and amiloride-sensitive sodium channels in vascular and renal cells.Nature reviews. Nephrology · 2014Review
- The migratory capacity of human trophoblastic BeWo cells: effects of aldosterone and the epithelial sodium channel.The Journal of membrane biology · 2013Article
- Depolymerization of cortical actin inhibits UT-A1 urea transporter endocytosis but promotes forskolin-stimulated membrane trafficking.American journal of physiology. Cell physiology · 2012Article
- Amiloride-sensitive channels are a major contributor to mechanotransduction in mammalian muscle spindles.The Journal of physiology · 2010Article
- Cell migration in BeWo cells and the role of epithelial sodium channels.The Journal of membrane biology · 2009Article
- Mechano-sensitivity of ENaC: may the (shear) force be with you.Pflugers Archiv : European journal of physiology · 2008Review
- Hypertonic saline attenuates colonic tumor cell metastatic potential by activating transmembrane sodium conductance.The Journal of membrane biology · 2006Article
- Regulation of sodium channel activity by capping of actin filaments.Molecular biology of the cell · 2003Article
- 2,3-butanedione monoxime (BDM), a potent inhibitor of actin-myosin interaction, induces ion and fluid transport in MDCK monolayers.Journal of muscle research and cell motility · 2002Article
- Actin modifies Ca2+ block of epithelial Na+ channels in planar lipid bilayers.Biophysical journal · 2001Article
- Ankyrin-Tiam1 interaction promotes Rac1 signaling and metastatic breast tumor cell invasion and migration.The Journal of cell biology · 2000Article
- Regulation of an inwardly rectifying ATP-sensitive K+ channel in the basolateral membrane of renal proximal tubule.The Journal of general physiology · 1998Article
- BNaC1 and BNaC2 constitute a new family of human neuronal sodium channels related to degenerins and epithelial sodium channels.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Disruption of actin filaments increases the activity of sodium-conducting channels in human myeloid leukemia cells.Molecular biology of the cell · 1996Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Amiloride-sensitive sodium channels are localized to the microvillar domain of apical membranes in sodium-transporting renal epithelial cells. To elucidate the elements that maintain sodium channel distribution at the apical membrane, we searched for specific proteins associating with the channel. Triton X-100 extraction of A6 epithelial cells reveals that sodium channels are associated with detergent-insoluble and assembled cytoskeleton. Indirect immunofluorescence and confocal microscopy show that sodium channels are segregated to the apical microvillar membrane and colocalize with ankyrin, fodrin, and actin. We document by immunoblot analysis that ankyrin and fodrin remain associated with sodium channels after isolation and purification from bovine renal papillae. 125I-labeled ankyrine can be precipitated by anti-sodium-channel antibodies only in the presence of purified bovine sodium-channel complex. Direct binding of 125I-labeled ankyrin shows ankyrin binds to the 150-kDa subunit of the channel. Fluorescence photobleach lateral-diffusion measurements indicate sodium channels are severely restricted in their lateral mobility. We conclude that ankyrin links the amiloride-sensitive sodium channel to the underlying cytoskeleton and this association may sequester sodium channels at apical microvilli and maintain their polarized distribution in renal epithelial cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.