ArticleBiophysical journal2006
Base of pore loop is important for rectification, activation, permeation, and block of Kir3.1/Kir3.4.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 8 citations in OpenAlex.
- Comparison of KHandbook of experimental pharmacology · 2021Article
- Cardiac strong inward rectifier potassium channels.Journal of molecular and cellular cardiology · 2010Review
- Blocker protection by short spermine analogs: refined mapping of the spermine binding site in a Kir channel.Biophysical journal · 2008Article
- Extracellular proton-modulated pore-blocking effect of the anticonvulsant felbamate on NMDA channels.Biophysical journal · 2007Article
- Galphai3 primes the G protein-activated K+ channels for activation by coexpressed Gbetagamma in intact Xenopus oocytes.The Journal of physiology · 2007Article
- Electrostatic interactions in the channel cavity as an important determinant of potassium channel selectivity.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Kir3.1/Kir3.4 channel is an inward rectifier, agonist-activated K(+) channel. The location of the binding site within the channel pore that coordinates polyamines (and is thus responsible for inward rectification) and the location of the gate that opens the channel in response to agonist activation is unclear. In this study, we show, not surprisingly, that mutation of residues at the base of the selectivity filter in the pore loop and second transmembrane domain weakens Cs(+) block and decreases selectivity (as measured by Rb(+) and spermine permeation). However, unexpectedly, the mutations also weaken inward rectification and abolish agonist activation of the channel. In the wild-type channel and 34 mutant channels, there are significant (p < 0.05) correlations among the K(D) for Cs(+) block, Rb(+) and spermine permeation, inward rectification, and agonist activation. The significance of these findings is discussed. One possible conclusion is that the selectivity filter is responsible for inward rectification and agonist activation as well as permeation and block.
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