ArticleJournal of bacteriology2006
Putative surface proteins encoded within a novel transferable locus confer a high-biofilm phenotype to Enterococcus faecalis.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed.
- Selection of fecal enterococci exhibiting tcrB-mediated copper resistance in pigs fed diets supplemented with copper.Applied and environmental microbiology · 2011Trial
- Virulence and Antibiotic Resistance Genes inMicroorganisms · 2025Article
- Novel approaches on root canal disinfection methods againstJournal of oral microbiology · 2025Review
- Review
- Current Knowledge of Enterococcal Endocarditis: A Disease Lurking in Plain Sight of Health Providers.Pathogens (Basel, Switzerland) · 2024Review
- Bridging Molecular and Clinical Sciences to Achieve the Best Treatment ofMicroorganisms · 2023Review
- Review
- New Insights into the Virulence Traits and Antibiotic Resistance of Enterococci Isolated from Diverse Probiotic Products.Microorganisms · 2021Article
- Effects of ClpP protease on biofilm formation of Enterococcus faecalis.Journal of applied oral science : revista FOB · 2021Article
- Plasmid Transfer by Conjugation in Gram-Negative Bacteria: From the Cellular to the Community Level.Genes · 2020Review
- Enterococci: Between Emerging Pathogens and Potential Probiotics.BioMed research international · 2019Review
- Evaluation of the Effect of MTAD on Expression of Enterococcus faecalis Virulence Factors Considering the Role of Different Obturating Materials.Journal of dentistry (Tehran, Iran) · 2018Article
- Long-Term Colonization Dynamics of Enterococcus faecalis in Implanted Devices in Research Macaques.Applied and environmental microbiology · 2018Article
- Distribution of antimicrobial resistance determinants, virulence-associated factors and clustered regularly interspaced palindromic repeats loci in isolates of Enterococcus faecalis from various settings and genetic lineages.Pathogens and disease · 2017Article
- Plasmids from Food Lactic Acid Bacteria: Diversity, Similarity, and New Developments.International journal of molecular sciences · 2015Review
- Enterococcus faecalis infection activates phosphatidylinositol 3-kinase signaling to block apoptotic cell death in macrophages.Infection and immunity · 2014Article
- Genomic comparative analysis of the environmental Enterococcus mundtii against enterococcal representative species.BMC genomics · 2014Article
- Role of house flies in the ecology of Enterococcus faecalis from wastewater treatment facilities.Microbial ecology · 2014Article
- The majority of a collection of U.S. endocarditis Enterococcus faecalis isolates obtained from 1974 to 2004 lack capsular genes and belong to diverse, non-hospital-associated lineages.Journal of clinical microbiology · 2014Article
- Occurrence of the transferable copper resistance gene tcrB among fecal enterococci of U.S. feedlot cattle fed copper-supplemented diets.Applied and environmental microbiology · 2013Article
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3 authors.
Funding
Abstract
Enterococci are opportunistic pathogens and among the leading causes of nosocomial infections. Enterococcus faecalis, the dominant species among infection-derived isolates, has recently been recognized as capable of forming biofilms on abiotic surfaces in vitro as well as on indwelling medical devices. A few bacterial factors known to contribute to biofilm formation in E. faecalis have been characterized. To identify additional factors which may be important to this process, we utilized a Tn917-based insertional mutagenesis strategy to generate a mutant bank in a high-biofilm-forming E. faecalis strain, E99. The resulting mutant bank was screened for mutants exhibiting a significantly reduced ability to form biofilms. One mutant, P101D12, which showed greater than 70% reduction in its ability to form biofilms compared to the wild-type parent, was further characterized. The single Tn917 insertion in P101D12 was mapped to a gene, bee-2, encoding a probable cell wall-anchored protein. Sequence information for the region flanking bee-2 revealed that this gene was a member of a locus (termed the bee locus for biofilm enhancer in enterococcus) comprised of five genes encoding three putative cell wall-anchored proteins and two probable sortases. Contour-clamped homogeneous electric field gel and Southern hybridization analyses suggested that the bee locus is likely harbored on a large conjugative plasmid. Filter mating assays using wild-type E99 or mutant P101D12 as a donor confirmed that the bee locus could transfer conjugally at high frequency to recipient E. faecalis strains. This represents the first instance of the identification of a mobile genetic element conferring biofilm-forming property in E. faecalis.
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