ArticleBMC developmental biology2006
Aberrant stalk development and breakdown of tip dominance in Dictyostelium cell lines with RNAi-silenced expression of calcineurin B.
Article in BMC developmental biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 19 citations in OpenAlex.
- Updating Insights into the Regulatory Mechanisms of Calcineurin-Activated Transcription Factor Crz1 in Pathogenic Fungi.Journal of fungi (Basel, Switzerland) · 2022Review
- Article
- The Dictyostelium prestalk inducer differentiation-inducing factor-1 (DIF-1) triggers unexpectedly complex global phosphorylation changes.Molecular biology of the cell · 2015Article
- A Simple Retroelement Based Knock-Down System in Dictyostelium: Further Insights into RNA Interference Mechanisms.PloS one · 2015Article
- Calcineurin-Crz1 signaling in lower eukaryotes.Eukaryotic cell · 2014Review
- RNAi silenced Dd-grp94 (Dictyostelium discoideum glucose-regulated protein 94 kDa) cell lines in Dictyostelium exhibit marked reduction in growth rate and delay in development.Gene expression · 2010Article
- Overexpression of calcineurin B subunit (CnB) enhances the oncogenic potential of HEK293 cells.Cancer science · 2008Article
- The inositol 1,4,5-trisphosphate receptor is required to signal autophagic cell death.Molecular biology of the cell · 2008Article
- Treasure hunt in an amoeba: non-coding RNAs in Dictyostelium discoideum.Current genetics · 2007Review
- The small RNA repertoire of Dictyostelium discoideum and its regulation by components of the RNAi pathway.Nucleic acids research · 2007Article
- Calcineurin controls growth, morphology, and pathogenicity in Aspergillus fumigatus.Eukaryotic cell · 2006Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCalcineurin, the Ca2+/calmodulin-dependent protein phosphatase, plays important roles in various cellular processes in lower and higher eukaryotes. Here we analyze the role of calcineurin in the development of Dictyostelium discoideum by RNAi-mediated manipulation of its expression.
resultsThe cnbA gene of Dictyostelium discoideum which encodes the regulatory B subunit (CNB) of calcineurin was silenced by RNAi. We found a variety of silencing levels of CNB in different recombinant cell lines. Reduction of CNB expression in a given cell line was correlated with developmental aberrations. Cell lines with strongly reduced protein levels developed slower than wild type cells and formed short stalks and spore heads with additional tips. Formation of short stalks results from incomplete vacuolization of prestalk cells during terminal differentiation. Expression of the stalk-specific gene ecmB was reduced in mutant cells. Aberrant stalk development is a cell autonomous defect, whereas the breakdown of tip dominance can be prevented by the presence of as low as 10% wild type cells in chimeras.
conclusionSilencing of calcineurin B in Dictyostelium by expression of RNAi reveals an unexpected link between increased intracellular calcium levels, possibly triggered by the morphogen DIF, activation of calcineurin, and the terminal stage of morphogenesis.
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