ArticleMolecular biology of the cell2006
Motion matters: secretory granule motion adjacent to the plasma membrane and exocytosis.
Article in Molecular biology of the cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 65 citations in OpenAlex.
- VAMP2 and synaptotagmin mobility in chromaffin granule membranes: implications for regulated exocytosis.Molecular biology of the cell · 2022Article
- Roles for the SNAP25 linker domain in the fusion pore and a dynamic plasma membrane SNARE "acceptor" complex.The Journal of general physiology · 2020Article
- Nanomachinery Organizing Release at Neuronal and Ribbon Synapses.International journal of molecular sciences · 2019Review
- SNARE-Mediated Fusion of Single Chromaffin Granules with Pore-Spanning Membranes.Biophysical journal · 2019Article
- The high-affinity calcium sensor synaptotagmin-7 serves multiple roles in regulated exocytosis.The Journal of general physiology · 2018Review
- Absolute position total internal reflection microscopy with an optical tweezer.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Distinct actions of Rab3 and Rab27 GTPases on late stages of exocytosis of insulin.Traffic (Copenhagen, Denmark) · 2014Article
- Protein mobility within secretory granules.Biophysical journal · 2014Article
- Identification of pauses during formation of HIV-1 virus like particles.Biophysical journal · 2013Article
- Mathematical modeling of insulin secretion and the role of glucose-dependent mobilization, docking, priming and fusion of insulin granules.Journal of theoretical biology · 2013Article
- Article
- Imaging single endocytic events reveals diversity in clathrin, dynamin and vesicle dynamics.Traffic (Copenhagen, Denmark) · 2011Article
- Vesicle pools: lessons from adrenal chromaffin cells.Frontiers in synaptic neuroscience · 2011Article
- Imaging with total internal reflection fluorescence microscopy for the cell biologist.Journal of cell science · 2010Review
- Mapping dynamic protein interactions to insulin secretory granule behavior with TIRF-FRET.Biophysical journal · 2010Article
- Localized topological changes of the plasma membrane upon exocytosis visualized by polarized TIRFM.The Journal of cell biology · 2010Article
- Loose coupling between calcium channels and sites of exocytosis in chromaffin cells.The Journal of physiology · 2009Article
- Improved surface-patterned platinum microelectrodes for the study of exocytotic events.Analytical chemistry · 2009Article
- Dopamine and amphetamine rapidly increase dopamine transporter trafficking to the surface: live-cell imaging using total internal reflection fluorescence microscopy.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- On the role of intravesicular calcium in the motion and exocytosis of secretory organelles.Communicative & integrative biology · 2009Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Total internal reflection fluorescence microscopy was used to monitor changes in individual granule motions related to the secretory response in chromaffin cells. Because the motions of granules are very small (tens of nanometers), instrumental noise in the quantitation of granule motion was taken into account. ATP and Ca2+, both of which prime secretion before fusion, also affect granule motion. Removal of ATP in permeabilized cells causes average granule motion to decrease. Nicotinic stimulation causes a calcium-dependent increase in average granule motion. This effect is more pronounced for granules that undergo exocytosis than for those that do not. Fusion is not preceded by a reduction in mobility. Granules sometimes move 100 nm or more up to and within a tenth of a second before fusion. Thus, the jittering motion of granules adjacent to the plasma membrane is regulated by factors that regulate secretion and may play a role in secretion. Motion continues until shortly before fusion, suggesting that interaction of granule and plasma membrane proteins is transient. Disruption of actin dynamics did not significantly alter granule motion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.