ArticleBMC cell biology2006
Microtubules regulate disassembly of epithelial apical junctions.
Article in BMC cell biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 91 citations in OpenAlex.
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- Vitamin D receptor upregulates tight junction protein claudin-5 against colitis-associated tumorigenesis.Mucosal immunology · 2022Article
- Kinesins in Mammalian Spermatogenesis and Germ Cell Transport.Frontiers in cell and developmental biology · 2022Review
- Mutations in non-muscle myosin 2A disrupt the actomyosin cytoskeleton in Sertoli cells and cause male infertility.Developmental biology · 2021Article
- Impact of Docetaxel on blood-brain barrier function and formation of breast cancer brain metastases.Journal of experimental & clinical cancer research : CR · 2019Article
- Myosin IIA-mediated forces regulate multicellular integrity during vascular sprouting.Molecular biology of the cell · 2019Article
- Microtubule Motors in Establishment of Epithelial Cell Polarity.Cold Spring Harbor perspectives in biology · 2018Review
- The role of microtubules in the regulation of epithelial junctions.Tissue barriers · 2018Review
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- ACF7 regulates inflammatory colitis and intestinal wound response by orchestrating tight junction dynamics.Nature communications · 2017Article
- Rab11-FIP1 phosphorylation by MARK2 regulates polarity in MDCK cells.Cellular logistics · 2017Article
- KIF17 regulates RhoA-dependent actin remodeling at epithelial cell-cell adhesions.Journal of cell science · 2016Article
- Tight junction CLDN2 gene is a direct target of the vitamin D receptor.Scientific reports · 2015Article
- Actin grips: circular actin-rich cytoskeletal structures that mediate the wrapping of polymeric microfibers by endothelial cells.Biomaterials · 2015Article
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- Neutrophil-derived JAML inhibits repair of intestinal epithelial injury during acute inflammation.Mucosal immunology · 2014Article
- Microtubules are required for efficient epithelial tight junction homeostasis and restoration.American journal of physiology. Cell physiology · 2014Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
backgroundEpithelial tight junction (TJ) and adherens junction (AJ) form the apical junctional complex (AJC) which regulates cell-cell adhesion, paracellular permeability and cell polarity. The AJC is anchored on cytoskeletal structures including actin microfilaments and microtubules. Such cytoskeletal interactions are thought to be important for the assembly and remodeling of apical junctions. In the present study, we investigated the role of microtubules in disassembly of the AJC in intestinal epithelial cells using a model of extracellular calcium depletion.
resultsCalcium depletion resulted in disruption and internalization of epithelial TJs and AJs along with reorganization of perijunctional F-actin into contractile rings. Microtubules reorganized into dense plaques positioned inside such F-actin rings. Depolymerization of microtubules with nocodazole prevented junctional disassembly and F-actin ring formation. Stabilization of microtubules with either docetaxel or pacitaxel blocked contraction of F-actin rings and attenuated internalization of junctional proteins into a subapical cytosolic compartment. Likewise, pharmacological inhibition of microtubule motors, kinesins, prevented contraction of F-actin rings and attenuated disassembly of apical junctions. Kinesin-1 was enriched at the AJC in cultured epithelial cells and it also accumulated at epithelial cell-cell contacts in normal human colonic mucosa. Furthermore, immunoprecipitation experiments demonstrated association of kinesin-1 with the E-cadherin-catenin complex.
conclusionOur data suggest that microtubules play a role in disassembly of the AJC during calcium depletion by regulating formation of contractile F-actin rings and internalization of AJ/TJ proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.