ArticleMolecular and cellular biology2006
Distinct mechanisms control the stability of the related S-phase cyclins Clb5 and Clb6.
Article in Molecular and cellular biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- Mitotic kinase oscillation governs the latching of cell cycle switches.Current biology : CB · 2022Article
- The S-Phase Cyclin Clb5 Promotes rRNA Gene (rDNA) Stability by Maintaining Replication Initiation Efficiency in rDNA.Molecular and cellular biology · 2021Article
- Purification of Cyclin-Dependent Kinase Fusion Complexes for In Vitro Analysis.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Article
- A comprehensive, mechanistically detailed, and executable model of the cell division cycle in Saccharomyces cerevisiae.Nature communications · 2019Article
- Cdk1 gates cell cycle-dependent tRNA synthesis by regulating RNA polymerase III activity.Nucleic acids research · 2018Article
- A Mad2-Mediated Translational Regulatory Mechanism Promoting S-Phase Cyclin Synthesis Controls Origin Firing and Survival to Replication Stress.Molecular cell · 2018Article
- Clb6-Cdc28 Promotes Ribonucleotide Reductase Subcellular Redistribution during S Phase.Molecular and cellular biology · 2018Article
- Building a Regulatory Network with Short Linear Sequence Motifs: Lessons from the Degrons of the Anaphase-Promoting Complex.Molecular cell · 2016Review
- The interplay between chromosome stability and cell cycle control explored through gene-gene interaction and computational simulation.Nucleic acids research · 2016Article
- Essential Roles of the Smc5/6 Complex in Replication through Natural Pausing Sites and Endogenous DNA Damage Tolerance.Molecular cell · 2015Article
- The Ubp15 deubiquitinase promotes timely entry into S phase in Saccharomyces cerevisiae.Molecular biology of the cell · 2015Article
- Redundancy or specificity? The role of the CDK Pho85 in cell cycle control.International journal of biochemistry and molecular biology · 2013Review
- Drosophila SUUR protein associates with PCNA and binds chromatin in a cell cycle-dependent manner.Chromosoma · 2013Article
- Defective in mitotic arrest 1 (Dma1) ubiquitin ligase controls G1 cyclin degradation.The Journal of biological chemistry · 2013Article
- Molecular basis of the functional distinction between Cln1 and Cln2 cyclins.Cell cycle (Georgetown, Tex.) · 2012Article
- Parametric modeling of cellular state transitions as measured with flow cytometry.BMC bioinformatics · 2012Article
- Cyclin-dependent kinases are regulators and effectors of oscillations driven by a transcription factor network.Molecular cell · 2012Article
- Do replication forks control late origin firing in Saccharomyces cerevisiae?Nucleic acids research · 2012Article
- SCFCdc4 enables mating type switching in yeast by cyclin-dependent kinase-mediated elimination of the Ash1 transcriptional repressor.Molecular and cellular biology · 2011Article
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3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The yeast S-phase cyclins Clb5 and Clb6 are closely related proteins that are synthesized late in G1. Although often grouped together with respect to function, Clb5 and Clb6 exhibit differences in their ability to promote S-phase progression. DNA replication is significantly slowed in clb5Delta mutants but not in clb6Delta mutants. We have examined the basis for the differential functions of Clb5 and Clb6 and determined that unlike Clb5, which is stable until mitosis, Clb6 is degraded rapidly at the G1/S border. N-terminal deletions of CLB6 were hyperstabilized, suggesting that the sequences responsible for directing the destruction of Clb6 reside in the N terminus. Clb6 lacks the destruction box motif responsible for the anaphase promoting complex-mediated destruction of Clb5 but contains putative Cdc4 degron motifs in the N terminus. Clb6 was hyperstabilized in cdc34-3 and cdc4-3 mutants at restrictive temperatures and when S/T-P phosphorylation sites in the N terminus were mutated to nonphosphorylatable residues. Efficient degradation of Clb6 requires the activities of both Cdc28 and Pho85. Finally, hyperstabilized Clb6 expressed from the CLB6 promoter rescued the slow S-phase defect exhibited by clb5Delta cells. Taken together, these findings suggest that the SCF(Cdc4) ubiquitin ligase complex regulates Clb6 turnover and that the functional differences exhibited by Clb5 and Clb6 arise from the distinct mechanisms controlling their stability.
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