ArticleMolecular and cellular biology2006
PB1 domain-dependent signaling complex is required for extracellular signal-regulated kinase 5 activation.
Article in Molecular and cellular biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 49 citations in OpenAlex.
- Structural basis for MEKK2 dimerization and substrate recognition.Nature communications · 2025Article
- Neuropathy-causing mutations in HSPB1 impair autophagy by disturbing the formation of SQSTM1/p62 bodies.Autophagy · 2019Article
- Prediction of Protein-Protein Interactions Based on Domain.Computational and mathematical methods in medicine · 2019Article
- The decrease in histone methyltransferase EZH2 in response to fluid shear stress alters endothelial gene expression and promotes quiescence.Angiogenesis · 2016Article
- Interaction with the Paxillin LD1 Motif Relieves MEKK2 Auto-inhibition.Journal of molecular signaling · 2015Article
- The PB1 domain in auxin response factor and Aux/IAA proteins: a versatile protein interaction module in the auxin response.The Plant cell · 2015Review
- Article
- Formation of chimeric genes by copy-number variation as a mutational mechanism in schizophrenia.American journal of human genetics · 2013Article
- MEKK2 kinase association with 14-3-3 protein regulates activation of c-Jun N-terminal kinase.The Journal of biological chemistry · 2013Article
- MEK and the inhibitors: from bench to bedside.Journal of hematology & oncology · 2013Review
- Structural mechanism for the specific assembly and activation of the extracellular signal regulated kinase 5 (ERK5) module.The Journal of biological chemistry · 2013Article
- MEK5/ERK5 pathway: the first fifteen years.Biochimica et biophysica acta · 2012Review
- Molecular basis of lysophosphatidic acid-induced NF-κB activation.Cellular signalling · 2010Review
- Mitogen-activated protein kinase signaling in the heart: angels versus demons in a heart-breaking tale.Physiological reviews · 2010Review
- Erk5 activation elicits a vasoprotective endothelial phenotype via induction of Kruppel-like factor 4 (KLF4).The Journal of biological chemistry · 2010Article
- CHIP-dependent termination of MEKK2 regulates temporal ERK activation required for proper hyperosmotic response.The EMBO journal · 2010Article
- PB1 domain interaction of p62/sequestosome 1 and MEKK3 regulates NF-kappaB activation.The Journal of biological chemistry · 2010Article
- MEK5 and ERK5 are mediators of the pro-myogenic actions of IGF-2.Journal of cell science · 2009Article
- Noncanonical function of MEKK2 and MEK5 PB1 domains for coordinated extracellular signal-regulated kinase 5 and c-Jun N-terminal kinase signaling.Molecular and cellular biology · 2007Article
- MAP kinases and the control of nuclear events.Oncogene · 2007Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
MEKK2, MEK5, and extracellular signal-regulated kinase 5 (ERK5) are members of a three-kinase cascade for the activation of ERK5. MEK5 is the only MAP2K to express a PB1 domain, and we have shown that it heterodimerizes with the PB1 domain of MEKK2. Here we demonstrate the MEK5 PB1 domain is a scaffold that also binds ERK5, functionally forming a MEKK2-MEK5-ERK5 complex. Reconstitution assays and CFP/YFP imaging (fluorescence resonance energy transfer [FRET]) measuring YFP-MEKK2/CFP-MEK5 and CFP-MEK5/YFP-ERK5 interactions define distinct MEK5 PB1 domain binding sites for MEKK2 and ERK5, with a C-terminal extension of the PB1 domain contributing to ERK5 binding. Stimulus-dependent CFP/YFP FRET in combination with mutational analysis was used to define MEK5 PB1 domain residues critical for the interaction of MEKK2/MEK5 and MEK5/ERK5 required for activation of the ERK5 pathway in living cells. Fusion of the MEK5 PB1 domain to the N terminus of MEK1 confers ERK5 regulation by a MAP2K normally regulating only ERK1/2. The MEK5 PB1 domain confers stringent MAP3K regulation of ERK5 relative to more promiscuous MAP3K control of ERK1/2, JNK, and p38.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.