ArticleBMC biochemistry2006
Farnesylation or geranylgeranylation? Efficient assays for testing protein prenylation in vitro and in vivo.
Article in BMC biochemistry, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 37 citations in OpenAlex.
- Farnesyltransferase Enables Modular Assembly of Dual-Functional Nanobody Conjugates for in Vivo Imaging of Inflammation.Angewandte Chemie (International ed. in English) · 2026Article
- Tissue Washing Improves Native Ambient Mass Spectrometry Detection of Membrane Proteins Directly from Tissue.Journal of the American Chemical Society · 2023Article
- Protective effects of intrathecal injection of AAV9-RabGGTB-GFPFrontiers in aging neuroscience · 2023Article
- GGTase3 is a newly identified geranylgeranyltransferase targeting a ubiquitin ligase.Nature structural & molecular biology · 2019Article
- Transcriptome analysis of finger millet (Journal of genetics · 2019Article
- Metabolic Labeling with an Alkyne-modified Isoprenoid Analog Facilitates Imaging and Quantification of the Prenylome in Cells.ACS chemical biology · 2016Article
- Selective Gas-Phase Oxidation and Localization of Alkylated Cysteine Residues in Polypeptide Ions via Ion/Ion Chemistry.Journal of proteome research · 2016Article
- Nuclear import of a lipid-modified transcription factor: mobilization of NFAT5 isoform a by osmotic stress.Cell cycle (Georgetown, Tex.) · 2011Article
- Measurement of protein farnesylation and geranylgeranylation in vitro, in cultured cells and in biopsies, and the effects of prenyl transferase inhibitors.Nature protocols · 2011Article
- A novel testis-specific GTPase serves as a link to proteasome biogenesis: functional characterization of RhoS/RSA-14-44 in spermatogenesis.Molecular biology of the cell · 2010Article
- A novel approach to tag and identify geranylgeranylated proteins.Electrophoresis · 2009Article
- Direct spatial control of Epac1 by cyclic AMP.Molecular and cellular biology · 2009Article
- Analysis of the eukaryotic prenylome by isoprenoid affinity tagging.Nature chemical biology · 2009Article
- Membrane-associated farnesylated UCH-L1 promotes alpha-synuclein neurotoxicity and is a therapeutic target for Parkinson's disease.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Towards complete sets of farnesylated and geranylgeranylated proteins.PLoS computational biology · 2007Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAvailable in vitro and in vivo methods for verifying protein substrates for posttranslational modifications via farnesylation or geranylgeranylation (for example, autoradiography with 3H-labeled anchor precursors) are time consuming (weeks/months), laborious and suffer from low sensitivity.
resultsWe describe a new technique for detecting prenyl anchors in N-terminally glutathione S-transferase (GST)-labeled constructs of target proteins expressed in vitro in rabbit reticulocyte lysate and incubated with 3H-labeled anchor precursors. Alternatively, hemagglutinin (HA)-labeled constructs expressed in vivo (in cell culture) can be used. For registration of the radioactive marker, we propose to use a thin layer chromatography (TLC) analyzer. As a control, the protein yield is tested by Western blotting with anti-GST- (or anti-HA-) antibodies on the same membrane that has been previously used for TLC-scanning. These protocols have been tested with Rap2A, v-Ki-Ras2 and RhoA (variant RhoA63L) including the necessary controls. We show directly that RasD2 is a farnesylation target.
conclusionSavings in time for experimentation and the higher sensitivity for detecting 3H-labeled lipid anchors recommend the TLC-scanning method with purified GST- (or HA-) tagged target proteins as the method of choice for analyzing their prenylation capabilities in vitro and in vivo and, possibly, also for studying the myristoyl and palmitoyl posttranslational modifications.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.