ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2006
Dissociation (Ds) constructs, mapped Ds launch pads and a transiently-expressed transposase system suitable for localized insertional mutagenesis in rice.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 53 citations in OpenAlex.
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- Transposable elements employ distinct integration strategies with respect to transcriptional landscapes in eukaryotic genomes.Nucleic acids research · 2020Article
- Insertional Mutagenesis Approaches and Their Use in Rice for Functional Genomics.Plants (Basel, Switzerland) · 2019Review
- Remusatia vivipara lectin and Sclerotium rolfsii lectin interfere with the development and gall formation activity of Meloidogyne incognita in transgenic tomato.Transgenic research · 2019Article
- Mutagenesis in Rice: The Basis for Breeding a New Super Plant.Frontiers in plant science · 2019Review
- Lack of Genotype and Phenotype Correlation in a Rice T-DNA Tagged Line Is Likely Caused by Introgression in the Seed Source.PloS one · 2016Article
- Transposon based activation tagging in diploid strawberry and monoploid derivatives of potato.Plant cell reports · 2014Article
- International Consortium of Rice Mutagenesis: resources and beyond.Rice (New York, N.Y.) · 2013Article
- Loss of Cellulose synthase-like F6 function affects mixed-linkage glucan deposition, cell wall mechanical properties, and defense responses in vegetative tissues of rice.Plant physiology · 2012Article
- Mutagenesis of barley malting quality QTLs with Ds transposons.Functional & integrative genomics · 2012Article
- SNP discovery and genetic mapping of T-DNA insertional mutants in Fragaria vesca L.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2010Article
- Over-expression of miR172 causes loss of spikelet determinacy and floral organ abnormalities in rice (Oryza sativa).BMC plant biology · 2009Article
- Mutant resources in rice for functional genomics of the grasses.Plant physiology · 2009Article
- Analysis of gene-trap Ds rice populations in Korea.Plant molecular biology · 2007Article
- A rice phenomics study--phenotype scoring and seed propagation of a T-DNA insertion-induced rice mutant population.Plant molecular biology · 2007Article
- A barley activation tagging system.Plant molecular biology · 2007Article
- Compact shoot and leafy head 1, a mutation affects leaf initiation and developmental transition in rice (Oryza sativa L).Plant cell reports · 2007Article
Corrections and comments
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Authors and funding
13 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a transiently-expressed transposase (TET)-mediated Dissociation (Ds) insertional mutagenesis system for generating stable insertion lines in rice which will allow localized mutagenesis of a chromosomal region. In this system, a Ds containing T-DNA construct was used to produce Ds launch pad lines. Callus tissues, from single-copy Ds/T-DNA lines, were then transiently infected with Agrobacterium harbouring an immobile Ac (iAc) construct, also containing a green fluorescent protein gene (sgfpS65T) as the visual marker. We have regenerated stable Ds insertion lines at a frequency of 9-13% using selection for Ds excision and GFP counter selection against iAc and nearly half of them were unique insertion lines. Double transformants (iAc/Ds) were also obtained and their progeny yielded approximately 10% stable insertion lines following excision and visual marker screening with 50% redundancy. In general, more than 50% of the Ds reinsertions were within 1 cM of the launch pad. We have produced a large number of single-copy Ds/T-DNA launch pads distributed over the rice chromosomes and have further refined the Ds/T-DNA construct to enrich for "clean" single-copy T-DNA insertions. The availability of single copy "clean" Ds/T-DNA launch pads will facilitate chromosomal region-directed insertion mutagenesis. This system provides an opportunity for distribution of gene tagging tasks among collaborating laboratories on the basis of chromosomal locations.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.