ArticleCell regulation1991
Guanosine 5'-thiotriphosphate may stimulate phosphoinositide messenger production in sea urchin eggs by a different route than the fertilizing sperm.
Article in Cell regulation, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 77 citations in OpenAlex.
- Guanine nucleotides in the meiotic maturation of starfish oocytes: regulation of the actin cytoskeleton and of Ca(2+) signaling.PloS one · 2009Article
- Calcium signalling in early embryos.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2008Review
- Calcium at fertilization and in early development.Physiological reviews · 2006Review
- Calcium release at fertilization in starfish eggs is mediated by phospholipase Cgamma.The Journal of cell biology · 1997Article
- Local perinuclear calcium signals associated with mitosis-entry in early sea urchin embryos.The Journal of cell biology · 1996Article
- Direct membrane retrieval into large vesicles after exocytosis in sea urchin eggs.The Journal of cell biology · 1995Article
- Cyclic ADP-ribose: a calcium mobilizing metabolite of NAD+.Molecular and cellular biochemistry · 1994Review
- The N-ethylmaleimide-sensitive protein thiol groups necessary for sea-urchin egg cortical-granule exocytosis are highly exposed to the medium and are required for triggering by Ca2+.The Biochemical journal · 1994Article
- Different triggers for calcium oscillations in mouse eggs involve a ryanodine-sensitive calcium store.The Biochemical journal · 1992Article
- Internal calcium release and activation of sea urchin eggs by cGMP are independent of the phosphoinositide signaling pathway.Molecular biology of the cell · 1992Article
- Identification of the sea urchin egg receptor for sperm using an antiserum raised against a fragment of its extracellular domain.The Journal of cell biology · 1992Article
- Organization of the sea urchin egg endoplasmic reticulum and its reorganization at fertilization.The Journal of cell biology · 1991Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We show that microinjecting guanosine-5'-thiotriphosphate (GTP gamma S) into unfertilized sea urchin eggs generates an intracellular free calcium concentration [( Ca]i) transient apparently identical in magnitude and duration to the calcium transient that activates the egg at fertilization. The GTP gamma S-induced transient is blocked by prior microinjection of the inositol trisphosphate (InsP3) antagonist heparin. GTP gamma S injection also causes stimulation of the egg's Na+/H+ antiporter via protein kinase C, even in the absence of a [Ca]i increase. These data suggest that GTP gamma S acts by stimulating the calcium-independent production of the phosphoinositide messengers InsP3 and diacylglycerol (DAG). However, the fertilization [Ca]i transient is not affected by heparin, nor can the sperm cause calcium-independent stimulation of protein kinase C. It seems that the bulk of InsP3 and DAG production at fertilization is triggered by the [Ca]i transient, not by the sperm itself. GDP beta S, a G-protein antagonist, does not affect the fertilization [Ca]i transient. Our findings do not support the idea that signal transduction at fertilization operates via a G-protein linked directly to a plasma membrane sperm receptor.
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