ArticleProceedings of the National Academy of Sciences of the United States of America2006
Delayed testicular aging in pituitary adenylate cyclase-activating peptide (PACAP) null mice.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 50 citations in OpenAlex.
- Age-related decline in behavior and reproductive health in male mice.Scientific reports · 2025Article
- The hypothalamic effects of PACAP on the hypothalamic-pituitary-gonadal axis in male mice.Frontiers in endocrinology · 2025Article
- Effects of the Neuropeptides Pituitary Adenylate Cyclase Activating Polypeptide and Vasoactive Intestinal Peptide in Male Fertility.Medicina (Kaunas, Lithuania) · 2024Article
- Seasonal changes in the expression of PACAP, VPAC1, VPAC2, PAC1 and testicular activity in the testis of the muskrat (<em>Ondatra zibethicus</em>).European journal of histochemistry : EJH · 2022Article
- Effects of Pituitary Adenylate Cyclase Activating Polypeptide on Cell Death.International journal of molecular sciences · 2022Review
- Sirt1 and Nrf2: regulation of Leydig cell oxidant/antioxidant intracellular environment and steroid formation†.Biology of reproduction · 2021Article
- PACAP: A regulator of mammalian reproductive function.Molecular and cellular endocrinology · 2020Review
- Alzheimer's Disease Mouse as a Model of Testis Degeneration.International journal of molecular sciences · 2020Article
- Protective Effects of PACAP in Peripheral Organs.Frontiers in endocrinology · 2020Review
- PACAP deficiency as a model of aging.GeroScience · 2018Review
- Immunomodulatory Roles of PACAP and VIP: Lessons from Knockout Mice.Journal of molecular neuroscience : MN · 2018Review
- The Effects of Aging and Sex Steroid Deficiency on the Murine Skeleton Are Independent and Mechanistically Distinct.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2017Article
- Examination of PACAP-Like Immunoreactivity in Urogenital Tumor Samples.Journal of molecular neuroscience : MN · 2016Article
- Knockout of the transcription factor Nrf2: Effects on testosterone production by aging mouse Leydig cells.Molecular and cellular endocrinology · 2015Article
- Article
- Serum Levels of Melatonin and Oxidative Stress Markers and Correlation between Them in Infertile Men.Journal of caring sciences · 2013Article
- Effects of pituitary adenylate cyclase activating polypeptide on human sperm motility.Journal of molecular neuroscience : MN · 2012Article
- Changes in primary lymphoid organs with aging.Seminars in immunology · 2012Review
- PACAP, an autocrine/paracrine regulator of gonadotrophs.Biology of reproduction · 2011Review
- Expression analysis of PAC1-R and PACAP genes in zebrafish embryos.Journal of molecular neuroscience : MN · 2011Article
Corrections and comments
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Authors and funding
9 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Age-related decline in male sex hormones is a direct consequence of testicular aging. These changes in the hormonal complement cause physiological disturbances affecting the quality of life for millions of aging men. To assess the influence on testicular aging of pituitary adenylate cyclase-activating peptide (PACAP), a polypeptide that regulates testicular steroidogenesis in vitro, we compared the testicular structure and function between C57BL/6 wild-type and PACAP-/- male mice, at 4 and 15 months of age. We show that, in 4-month-old PACAP-/- mice, steroidogenesis (evaluated by levels of testosterone, steroidogenic acute regulatory protein, 3beta-hydroxysteroid dehydrogenase, and P450c17) was impaired. However, the testicular structure of these animals was not affected. At 15 months of age, wild-type testis displayed typical signs of aging (patchy seminiferous tubules, germ cell depletion, and vacuolization), whereas testicular structure was remarkably well conserved in PACAP-/- animals. The depletion of germ cells found in wild-type animals was associated with a higher content of peroxynitrites, a marker of reactive oxygen species, and a higher number of apoptotic cells compared with PACAP-/- mice. Our results show that testicular aging is delayed in PACAP-/- animals. Because the expression levels of steroidogenic factors are low and constant over time in knockout animals, a proposed mechanism for the protection against testicular degeneration is that production of reactive oxygen species, a byproduct of steroidogenesis that induces apoptosis, is down-regulated in PACAP-/- animals.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.