ArticleThe Journal of general physiology2006
On the interaction of neomycin with the slow vacuolar channel of Arabidopsis thaliana.
Article in The Journal of general physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 38 citations in OpenAlex.
- The Two-Pore Channel 2 in Human Physiology and Diseases: Functional Characterisation and Pharmacology.International journal of molecular sciences · 2025Review
- Electrophysiology and fluorescence to investigate cation channels and transporters in isolated plant vacuoles.Stress biology · 2022Review
- Current Methods to Unravel the Functional Properties of Lysosomal Ion Channels and Transporters.Cells · 2022Review
- Review
- Beneficial effects of naringenin in liver diseases: Molecular mechanisms.World journal of gastroenterology · 2018Review
- Naringenin Impairs Two-Pore Channel 2 Activity And Inhibits VEGF-Induced Angiogenesis.Scientific reports · 2017Article
- The human two-pore channel 1 is modulated by cytosolic and luminal calcium.Scientific reports · 2017Article
- The phosphoinositide PI(3,5)P₂ mediates activation of mammalian but not plant TPC proteins: functional expression of endolysosomal channels in yeast and plant cells.Cellular and molecular life sciences : CMLS · 2014Article
- Neomycin inhibition of (+)-7-iso-jasmonoyl-L-isoleucine accumulation and signaling.Journal of chemical ecology · 2014Article
- Plant salt stress status is transmitted systemically via propagating calcium waves.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Modulation of plant TPC channels by polyunsaturated fatty acids.Journal of experimental botany · 2012Article
- The Arabidopsis central vacuole as an expression system for intracellular transporters: functional characterization of the Cl-/H+ exchanger CLC-7.The Journal of physiology · 2012Article
- The putative K(+) channel subunit AtKCO3 forms stable dimers in Arabidopsis.Frontiers in plant science · 2012Article
- Aminoglycoside antibiotics: structure, functions and effects on in vitro plant culture and genetic transformation protocols.Plant cell reports · 2010Review
- Plant ion channels: gene families, physiology, and functional genomics analyses.Annual review of physiology · 2009Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study investigates the interaction of the aminoglycoside antibiotic neomycin with the slow vacuolar (SV) channel in vacuoles from Arabidopsis thaliana mesophyll cells. Patch-clamp experiments in the excised patch configuration revealed a complex pattern of neomycin effects on the channel: applied at concentrations in the submicromolar to millimolar range neomycin (a) blocked macroscopic SV currents in a voltage- and concentration-dependent manner, (b) slowed down activation and deactivation kinetics of the channel, and most interestingly, (c) at concentrations above 10 muM, neomycin shifted the SV activation threshold towards negative membrane potentials, causing a two-phasic activation at high concentrations. Single channel experiments showed that neomycin causes these macroscopic effects by combining a decrease of the single channel conductance with a concomitant increase of the channel's open probability. Our results clearly demonstrate that the SV channel can be activated at physiologically relevant tonoplast potentials in the presence of an organic effector molecule. We therefore propose the existence of a cellular equivalent regulating the activity of the SV channel in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.