ArticleBMC microbiology2006
Molecular targets for rapid identification of Brucella spp.
Article in BMC microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 52 citations in OpenAlex.
- Beyond Serology: A Meta-Analysis of Advancements in Molecular Detection of Brucella spp. in Seronegative Animals and Biological Samples.Veterinary medicine and science · 2025Pooled it
- Rapid detection of Brucella cells using a gold nanoparticle-based aptasensor via a simple colorimetric method.BMC veterinary research · 2024Article
- Article
- Review
- Whole-Genome Sequencing for Tracing the Genetic Diversity ofPathogens (Basel, Switzerland) · 2021Article
- Genome-scale approach to study the genetic relatedness among Brucella melitensis strains.PloS one · 2020Article
- Specific Detection and Differentiation BetweenFrontiers in veterinary science · 2020Article
- The Role of the Flagellar Protein FlgJ in the Virulence ofFrontiers in cellular and infection microbiology · 2020Article
- Laboratory Diagnosis of Human Brucellosis.Clinical microbiology reviews · 2019Review
- Multivalent Fusion DNA Vaccine againstBioMed research international · 2017Article
- Swine brucellosis: current perspectives.Veterinary medicine (Auckland, N.Z.) · 2017Review
- Immunogenicity of a Multi-Epitope DNA Vaccine Encoding Epitopes from Cu-Zn Superoxide Dismutase and Open Reading Frames ofFrontiers in immunology · 2017Article
- Pyruvate kinase is necessary for Brucella abortus full virulence in BALB/c mouse.Veterinary research · 2016Article
- Complete genome-wide screening and subtractive genomic approach revealed new virulence factors, potential drug targets against bio-war pathogen Brucella melitensis 16M.Drug design, development and therapy · 2015Article
- Efficient diagnosis and treatment follow-up of human brucellosis by a novel quantitative TaqMan real-time PCR assay: a human clinical survey.Journal of clinical microbiology · 2014Article
- Lon Mutant of Brucella abortus Induces Tumor Necrosis Factor-Alpha in Murine J774.A1 Macrophage.Osong public health and research perspectives · 2013Article
- GenoSets: visual analytic methods for comparative genomics.PloS one · 2012Article
- Analyses of Brucella pathogenesis, host immunity, and vaccine targets using systems biology and bioinformatics.Frontiers in cellular and infection microbiology · 2012Review
- Characterization of genomic island 3 and genetic variability of Chilean field strains of Brucella abortus.Journal of clinical microbiology · 2011Article
- Whole-genome-based phylogeny and divergence of the genus Brucella.Journal of bacteriology · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBrucella is an intracellular pathogen capable of infecting animals and humans. There are six recognized species of Brucella that differ in their host preference. The genomes of the three Brucella species have been recently sequenced. Comparison of the three revealed over 98% sequence similarity at the protein level and enabled computational identification of common and differentiating genes. We validated these computational predictions and examined the expression patterns of the putative unique and differentiating genes, using genomic and reverse transcription PCR. We then screened a set of differentiating genes against classical Brucella biovars and showed the applicability of these regions in the design of diagnostic tests.
resultsWe have identified and tested set of molecular targets that are associated in unique patterns with each of the sequenced Brucella spp. A comprehensive comparison was made among the published genome sequences of B. abortus, B. melitensis and B. suis. The comparison confirmed published differences between the three Brucella genomes, and identified subsets of features that were predicted to be of interest in a functional comparison of B. melitensis and B. suis to B. abortus. Differentiating sequence regions from B. abortus, B. melitensis and B. suis were used to develop PCR primers to test for the existence and in vitro transcription of these genes in these species. Only B. suis is found to have a significant number of unique genes, but combinations of genes and regions that exist in only two out of three genomes and are therefore useful for diagnostics were identified and confirmed.
conclusionAlthough not all of the differentiating genes identified were transcribed under steady state conditions, a group of genes sufficient to discriminate unambiguously between B. suis, B. melitensis, and B. abortus was identified. We present an overview of these genomic differences and the use of these features to discriminate among a number of Brucella biovars.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.