ArticleOncogene2006
17-AAG, an Hsp90 inhibitor, causes kinetochore defects: a novel mechanism by which 17-AAG inhibits cell proliferation.
Article in Oncogene, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 68 citations in OpenAlex.
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- Native Size-Exclusion Chromatography-Based Mass Spectrometry Reveals New Components of the Early Heat Shock Protein 90 Inhibition Response Among Limited Global Changes.Molecular & cellular proteomics : MCP · 2023Article
- Effects of aneuploidy on cell behaviour and function.Nature reviews. Molecular cell biology · 2022Review
- Role of HSP90 in Cancer.International journal of molecular sciences · 2021Review
- CENP-A Ubiquitylation Is Indispensable to Cell Viability.Developmental cell · 2019Article
- Hsp90 Inhibitor SNX-2112 Enhances TRAIL-Induced Apoptosis of Human Cervical Cancer Cells via the ROS-Mediated JNK-p53-Autophagy-DR5 Pathway.Oxidative medicine and cellular longevity · 2019Article
- SGT1-HSP90 complex is required for CENP-A deposition at centromeres.Cell cycle (Georgetown, Tex.) · 2017Article
- Protein Quality Control by Molecular Chaperones in Neurodegeneration.Frontiers in neuroscience · 2017Review
- Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins.Journal of visualized experiments : JoVE · 2016Article
- The oncogenic role of the cochaperone Sgt1.Oncogenesis · 2015Article
- CENP-A K124 Ubiquitylation Is Required for CENP-A Deposition at the Centromere.Developmental cell · 2015Article
- Whole chromosome aneuploidy: big mutations drive adaptation by phenotypic leap.BioEssays : news and reviews in molecular, cellular and developmental biology · 2012Article
- Article
- Heat shock protein 90-mediated inactivation of nuclear factor-κB switches autophagy to apoptosis through becn1 transcriptional inhibition in selenite-induced NB4 cells.Molecular biology of the cell · 2011Article
- Multifaceted intervention by the Hsp90 inhibitor ganetespib (STA-9090) in cancer cells with activated JAK/STAT signaling.PloS one · 2011Article
- High-content, high-throughput analysis of cell cycle perturbations induced by the HSP90 inhibitor XL888.PloS one · 2011Article
- Hsp90 inhibition increases p53 expression and destabilizes MYCN and MYC in neuroblastoma.International journal of oncology · 2011Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
The Hsp90 inhibitor 17-allylaminogeldanamycin (17-AAG), which is currently in clinical trials, is thought to exert antitumor activity by simultaneously targeting several oncogenic signaling pathways. Here we report a novel mechanism by which 17-AAG inhibits cell proliferation, and we provide the first evidence that HSP90 is required for the assembly of kinetochore protein complexes in humans. 17-AAG caused delocalization of several kinetochore proteins including CENP-I and CENP-H but excluding CENP-B and CENP-C. Consistently, 17-AAG induced a mitotic arrest that depends on the spindle checkpoint and induced misalignment of chromosomes and aneuploidy. We found that HSP90 associates with SGT1 (suppressor of G2 allele of skp1; SUGT1) in human cells and that depletion of SGT1 sensitizes HeLa cells to 17-AAG. Overexpression of SGT1 restored the localization of specific kinetochore proteins and chromosome alignment in cells treated with 17-AAG. Biochemical and genetic results suggest that HSP90, through its interaction with SGT1 (SUGT1), is required for kinetochore assembly. Furthermore, time-course experiments revealed that transient treatment with 17-AAG between late S and G2/M phases causes substantial delocalization of CENP-H and CENP-I, a finding that strongly suggests that HSP90 participates in kinetochore assembly in a cell cycle-dependent manner.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.