ArticleJournal of virology2006
Suppression of viral RNA recombination by a host exoribonuclease.
Article in Journal of virology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed, 71 citations in OpenAlex.
- Comprehensive Analysis of Insertions in BVDV and Implications for Non-Homologous Recombination Mechanisms.Viruses · 2026Article
- Screening bacterial effectors and human virus proteins in yeast to identify host factors driving tombusvirus RNA recombination: a role for autophagy and membrane phospholipid content.Journal of virology · 2025Article
- Co-opted SUMO machinery promotes condensate formation associated with membranous replication organelles of a positive-strand RNA virus.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- The Recombination Potential between SARS-CoV-2 and MERS-CoV from Cross-Species Spill-over Infections.Journal of epidemiology and global health · 2021Article
- Interviral Recombination between Plant, Insect, and Fungal RNA Viruses: Role of the Intracellular CaJournal of virology · 2019Article
- Article
- Identification of phlebovirus and arenavirus RNA sequences that stall and repress the exoribonuclease XRN1.The Journal of biological chemistry · 2018Article
- Evaluation of a recombination-resistant coronavirus as a broadly applicable, rapidly implementable vaccine platform.Communications biology · 2018Article
- Nonreplicative RNA Recombination of an Animal Plus-Strand RNA Virus in the Absence of Efficient Translation of Viral Proteins.Genome biology and evolution · 2017Article
- XRN1 Is a Species-Specific Virus Restriction Factor in Yeasts.PLoS pathogens · 2016Article
- The proteasomal Rpn11 metalloprotease suppresses tombusvirus RNA recombination and promotes viral replication via facilitating assembly of the viral replicase complex.Journal of virology · 2015Article
- Coordinated function of cellular DEAD-box helicases in suppression of viral RNA recombination and maintenance of viral genome integrity.PLoS pathogens · 2015Article
- Tombusvirus-yeast interactions identify conserved cell-intrinsic viral restriction factors.Frontiers in plant science · 2014Review
- Inhibition and avoidance of mRNA degradation by RNA viruses.Current opinion in microbiology · 2012Review
- RNA structural elements determine frequency and sites of nonhomologous recombination in an animal plus-strand RNA virus.Journal of virology · 2012Article
- The dependence of viral RNA replication on co-opted host factors.Nature reviews. Microbiology · 2011Review
- Role of RNase MRP in viral RNA degradation and RNA recombination.Journal of virology · 2011Article
- Translation elongation factor 1A facilitates the assembly of the tombusvirus replicase and stimulates minus-strand synthesis.PLoS pathogens · 2010Article
- An RNA pseudoknot is required for production of yellow fever virus subgenomic RNA by the host nuclease XRN1.Journal of virology · 2010Article
- The combined effect of environmental and host factors on the emergence of viral RNA recombinants.PLoS pathogens · 2010Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA viruses of humans, animals, and plants evolve rapidly due to mutations and RNA recombination. A previous genome-wide screen in Saccharomyces cerevisiae, a model host, identified five host genes, including XRN1, encoding a 5'-3' exoribonuclease, whose absence led to an approximately 10- to 50-fold enhancement of RNA recombination in Tomato bushy stunt virus (E. Serviene, N. Shapka, C. P. Cheng, T. Panavas, B. Phuangrat, J. Baker, and P. D. Nagy, Proc. Natl. Acad. Sci. USA 102:10545-10550, 2005). In this study, we found abundant 5'-truncated viral RNAs in xrn1delta mutant strains but not in the parental yeast strains, suggesting that these RNAs might serve as recombination substrates promoting RNA recombination in xrn1delta mutant yeast. This model is supported by data showing that an enhanced level of viral recombinant accumulation occurred when two different 5'-truncated viral RNAs were expressed in the parental and xrn1delta mutant yeast strains or electroporated into plant protoplasts. Moreover, we demonstrate that purified Xrn1p can degrade the 5'-truncated viral RNAs in vitro. Based on these findings, we propose that Xrn1p can suppress viral RNA recombination by rapidly removing the 5'-truncated RNAs, the substrates of recombination, and thus reducing the chance for recombination to occur in the parental yeast strain. In addition, we show that the 5'-truncated viral RNAs are generated by host endoribonucleases. Accordingly, overexpression of the Ngl2p endoribonuclease led to an increased accumulation of cleaved viral RNAs in vivo and in vitro. Altogether, this paper establishes that host ribonucleases and host-mediated viral RNA turnover play major roles in RNA virus recombination and evolution.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.