ArticleBiophysical journal2006
Sub-angstrom conformational changes of a single molecule captured by AFM variance analysis.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Forcing a Molecule to Switch: Quantifying Mechanical Control at the Atomic Scale.Nano letters · 2026Article
- Molecular Fluctuations as a Ruler of Force-Induced Protein Conformations.Nano letters · 2021Article
- Article
- Data-driven techniques for detecting dynamical state changes in noisily measured 3D single-molecule trajectories.Molecules (Basel, Switzerland) · 2014Article
- Mechanochemistry: one bond at a time.ACS nano · 2009Article
- Mechanical characterization of protein L in the low-force regime by electromagnetic tweezers/evanescent nanometry.Biophysical journal · 2009Article
- Quantifying DNA melting transitions using single-molecule force spectroscopy.Journal of physics. Condensed matter : an Institute of Physics journal · 2009Article
- Quantifying multiscale noise sources in single-molecule time series.The journal of physical chemistry. B · 2009Article
- Extracting Kinetic and Stationary Distribution Information from Short MD Trajectories via a Collection of Surrogate Diffusion Models.Journal of chemical theory and computation · 2009Article
- Comment on "Sub-angstrom conformational changes of a single molecule captured by AFM variance analysis".Biophysical journal · 2008Article
- A fluorescence energy transfer-based mechanical stress sensor for specific proteins in situ.The FEBS journal · 2008Article
- Single-molecule biophysics: at the interface of biology, physics and chemistry.Journal of the Royal Society, Interface · 2008Review
- Conformational equilibria in monomeric alpha-synuclein at the single-molecule level.PLoS biology · 2008Article
- Force-clamp spectroscopy of single-protein monomers reveals the individual unfolding and folding pathways of I27 and ubiquitin.Biophysical journal · 2007Article
- Conformational transitions in single polymer molecules modeled with a complete energy landscape: continuous two-state model.The European physical journal. E, Soft matter · 2007Article
- Direct measurement of the full, sequence-dependent folding landscape of a nucleic acid.Science (New York, N.Y.) · 2006Article
- Simulating force-induced conformational transitions in polysaccharides with the SMD replica exchange method.Biophysical journal · 2006Article
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4 authors.
Funding
Abstract
A system's equilibrium variance can be analyzed to probe its underlying dynamics at higher resolution. Here, using single-molecule atomic-force microscope techniques, we show how the variance in the length of a single dextran molecule can be used to establish thermodynamic equilibrium and to detect conformational changes not directly observable with other methods. Dextran is comprised of a chain of pyranose rings that each undergoes an Angstrom-scale transition from a chair to boat conformation under a stretching force. Our analysis of the variance of the molecule's fluctuations verifies equilibrium throughout the force-extension curve, consistent with the expected thermodynamic ensemble. This validates further analysis of the variance in the transition region, which reveals an intermediate conformation between the chair and the boat on the sub-Angstrom scale. Our test of thermal equilibrium as well as our variance analysis can be readily extended to a wide variety of molecules, including proteins.
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