ArticleBiophysical journal2006
Carboxy terminus splice variation alters ClC channel gating and extracellular cysteine reactivity.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- ClC Channels and Transporters: Structure, Physiological Functions, and Implications in Human Chloride Channelopathies.Frontiers in pharmacology · 2017Review
- Role of CBS and Bateman Domains in Phosphorylation-Dependent Regulation of a CLC Anion Channel.Biophysical journal · 2016Article
- The voltage-gated anion channels encoded by clh-3 regulate egg laying in C. elegans by modulating motor neuron excitability.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- The ClC-3 chloride channel and osmoregulation in the European sea bass, Dicentrarchus labrax.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2013Article
- Regulatory phosphorylation induces extracellular conformational changes in a CLC anion channel.Biophysical journal · 2013Article
- CLC anion channel regulatory phosphorylation and conserved signal transduction domains.Biophysical journal · 2012Article
- C. elegans STK39/SPAK ortholog-mediated inhibition of ClC anion channel activity is regulated by WNK-independent ERK kinase signaling.American journal of physiology. Cell physiology · 2011Article
- ATP induces conformational changes in the carboxyl-terminal region of ClC-5.The Journal of biological chemistry · 2011Article
- Antiport mechanism for Cl(-)/H(+) in ClC-ec1 from normal-mode analysis.Biophysical journal · 2010Article
- Identification of regulatory phosphorylation sites in a cell volume- and Ste20 kinase-dependent ClC anion channel.The Journal of general physiology · 2009Article
- Putting the pieces together: a crystal clear window into CLC anion channel regulation.Channels (Austin, Tex.)Article
- Unique gating properties of C. elegans ClC anion channel splice variants are determined by altered CBS domain conformation and the R-helix linker.Channels (Austin, Tex.)Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
CLH-3a and CLH-3b are Caenorhabditis elegans ClC channel splice variants that exhibit striking differences in voltage, Cl(-), and H(+) sensitivity. The major primary structure differences between the channels include a 71 amino acid CLH-3a N-terminal extension and a 270 amino acid extension of the CLH-3b C-terminus. Deletion of the CLH-3a N-terminus or generation of a CLH-3a/b chimera has no effect on channel gating. In contrast, deletion of a 169 amino acid C-terminal CLH-3b splice insert or deletion of the last 11 amino acids of cystathionine-beta-synthase domain 1 gives rise to functional properties identical to those of CLH-3a. Voltage-, Cl(-)-, and H(+)-dependent gating of both channels are lost when their glutamate gates are mutated to alanine. Glutamate gate cysteine mutants exhibit similar degrees of inhibition by MTSET, but the inhibition time constant of CLH-3b is sevenfold greater than that of CLH-3a. Differences in MTSET inhibition are reversed by deletion of the same cytoplasmic C-terminal regions that alter CLH-3b gating. Our results indicate that splice variation of the CLH-3b cytoplasmic C-terminus alters extracellular structure and suggest that differences in the conformation of the outer pore vestibule and associated glutamate gate may account for differences in CLH-3a and CLH-3b gating.
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