ArticleNucleic acids research2006
The use of biotin tagging in Saccharomyces cerevisiae improves the sensitivity of chromatin immunoprecipitation.
Article in Nucleic acids research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 59 citations in OpenAlex.
- Modified pea apyrase has altered nuclear functions and enhances the growth of yeast and Arabidopsis.Frontiers in plant science · 2025Article
- Transcription factor-based biosensors for screening and dynamic regulation.Frontiers in bioengineering and biotechnology · 2023Review
- Rapid Synthesis of theACS omega · 2022Article
- Nonradioactive direct telomerase activity detection using biotin-labeled primers.Journal of clinical laboratory analysis · 2021Article
- Transcription levels of a noncoding RNA orchestrate opposing regulatory and cell fate outcomes in yeast.Cell reports · 2021Article
- Proteomic-based approaches to cardiac development and disease.Current opinion in chemical biology · 2019Review
- Isolation of secreted proteins from Drosophila ovaries and embryos through in vivo BirA-mediated biotinylation.PloS one · 2019Article
- Capturing the Asc1p/Molecular & cellular proteomics : MCP · 2017Article
- Article
- Evolution of reduced co-activator dependence led to target expansion of a starvation response pathway.eLife · 2017Article
- Utilizing Biotinylated Proteins Expressed in Yeast to Visualize DNA-Protein Interactions at the Single-Molecule Level.Frontiers in microbiology · 2017Article
- Article
- The Cardiac TBX5 Interactome Reveals a Chromatin Remodeling Network Essential for Cardiac Septation.Developmental cell · 2016Article
- Rapid isolation and single-molecule analysis of ribonucleoproteins from cell lysate by SNAP-SiMPull.RNA (New York, N.Y.) · 2015Article
- One-step enzymatic modification of the cell surface redirects cellular cytotoxicity and parasite tropism.ACS chemical biology · 2015Article
- Discrimination between thermodynamic models of cis-regulation using transcription factor occupancy data.Nucleic acids research · 2014Article
- Proteomic profiling of cardiac tissue by isolation of nuclei tagged in specific cell types (INTACT).Development (Cambridge, England) · 2014Article
- Recruitment of TREX to the transcription machinery by its direct binding to the phospho-CTD of RNA polymerase II.PLoS genetics · 2013Article
- Mapping functional transcription factor networks from gene expression data.Genome research · 2013Article
- Genome-wide measurement of protein-DNA binding dynamics using competition ChIP.Nature protocols · 2013Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Affinity tagging has been used in many global studies towards protein function. We describe a highly efficient system for in vivo biotinylation of transcription factors in the yeast Saccharomyces cerevisiae, which is based on the bacterial BirA biotin ligase. The strength of the biotin-streptavidin interaction was exploited to improve detection of in vivo protein-DNA complexes in chromatin immunoprecipitation (ChIP) experiments. In a test system using the biotin-tagged LexA DNA-binding protein, we found that stringent washing conditions resulted in a strong improvement of the signal-to-noise ratios. Yeast strains with chromosomally integrated versions of tagged transcription factor genes were generated using N- or C-terminal biotin-tagging cassettes. ChIP experiments with biotinylated Rbp3p, a RNA polymerase II subunit, showed that Rbp3p-binding could even be detected at weakly expressed genes. Other methods failed to detect RNA polymerase II binding at such genes. Our results show that biotinylation of yeast transcription factors improves the detection of in vivo protein-DNA complexes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.