ArticleThe Journal of comparative neurology2006
Development of gamma-aminobutyric acidergic synapses in cultured hippocampal neurons.
Article in The Journal of comparative neurology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Beta-Amyloid (AβMolecules (Basel, Switzerland) · 2022Article
- Leptin increases GABAergic synaptogenesis through the Rho guanine exchange factor β-PIX in developing hippocampal neurons.Science signaling · 2021Article
- Enhanced GABAergic actions resulting from the coapplication of the steroid 3α-hydroxy-5α-pregnane-11,20-dione (alfaxalone) with propofol or diazepam.Scientific reports · 2018Article
- Brain extracellular matrix retains connectivity in neuronal networks.Scientific reports · 2015Article
- Ontogeny of biochemical, morphological and functional parameters of synaptogenesis in primary cultures of rat hippocampal and cortical neurons.Molecular brain · 2015Article
- Pro-brain-derived neurotrophic factor inhibits GABAergic neurotransmission by activating endocytosis and repression of GABAA receptors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Brain-derived neurotrophic factor promotes gephyrin protein expression and GABAA receptor clustering in immature cultured hippocampal cells.Neurochemistry international · 2014Article
- GABAA receptor membrane insertion rates are specified by their subunit composition.Molecular and cellular neurosciences · 2013Article
- Homeostatic responses fail to correct defective amygdala inhibitory circuit maturation in fragile X syndrome.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- The possible role of GABAA receptors and gephyrin in epileptogenesis.Frontiers in cellular neuroscience · 2013Article
- γ-Aminobutyric acid type A (GABAA) receptor α subunits play a direct role in synaptic versus extrasynaptic targeting.The Journal of biological chemistry · 2012Article
- Diffusion barriers constrain receptors at synapses.PloS one · 2012Article
- Homeostatic strengthening of inhibitory synapses is mediated by the accumulation of GABA(A) receptors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Hydrophobic anions potently and uncompetitively antagonize GABA(A) receptor function in the absence of a conventional binding site.British journal of pharmacology · 2011Article
- Impact of synaptic neurotransmitter concentration time course on the kinetics and pharmacological modulation of inhibitory synaptic currents.Frontiers in cellular neuroscience · 2011Article
- Organization of amyloid-beta protein precursor intracellular domain-associated protein-1 in the rat brain.The Journal of comparative neurology · 2010Article
- Nitric oxide alters GABAergic synaptic transmission in cultured hippocampal neurons.Brain research · 2009Article
- Age- and gender-related differences in GABAA receptor-mediated postsynaptic currents in GABAergic neurons of the substantia nigra reticulata in the rat.Neuroscience · 2009Article
- A ketogenic diet rescues the murine succinic semialdehyde dehydrogenase deficient phenotype.Experimental neurology · 2008Article
- Subunit-specific trafficking of GABA(A) receptors during status epilepticus.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
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5 authors.
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Abstract
The formation and maturation of gamma-aminobutyric acid (GABA)-ergic synapses was studied in cultured hippocampal pyramidal neurons by both performing immunocytochemistry for GABAergic markers and recording miniature inhibitory postsynaptic currents (mIPSCs). Nascent GABAergic synapses appeared between 3 and 8 days in vitro (DIV), with GABAA receptor subunit clusters appearing first, followed by GAD-65 puncta, then functional synapses. The number of GABAergic synapses increased from 7 to 14 DIV, with a corresponding increase in frequency of mIPSCs. Moreover, these new GABAergic synapses formed on neuronal processes farther from the soma, contributing to decreased mIPSC amplitude and slowed mIPSC 19-90% rise time. The mIPSC decay quickened from 7 to 14 DIV, with a parallel change in the distribution of the alpha5 subunit from diffuse expression at 7 DIV to clustered expression at 14 DIV. These alpha5 clusters were mostly extrasynaptic. The alpha1 subunit was expressed as clusters in none of the neurons at 7 DIV, in 20% at 14 DIV, and in 80% at 21 DIV. Most of these alpha1 clusters were expressed at GABAergic synapses. In addition, puncta of GABA transporter 1 (GAT-1) were localized to GABAergic synapses at 14 DIV but were not expressed at 7 DIV. These studies demonstrate that mIPSCs appear after pre- and postsynaptic elements are in place. Furthermore, the process of maturation of GABAergic synapses involves increased synapse formation at distal processes, expression of new GABAA receptor subunits, and GAT-1 expression at synapses; these changes are reflected in altered frequency, kinetics, and drug sensitivity of mIPSCs.
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