ArticleGene therapy2006
Inducible regulation of Runx2-stimulated osteogenesis.
Article in Gene therapy, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 60 citations in OpenAlex.
- Intelligent design of tumor microenvironment-responsive Adeno-associated virus vectors: overcoming delivery barriers and enabling precision therapy.Medical oncology (Northwood, London, England) · 2025Review
- Applications of synthetic biology in medical and pharmaceutical fields.Signal transduction and targeted therapy · 2023Review
- Iron Oxide Nanoparticles: A Review on the Province of Its Compounds, Properties and Biological Applications.Materials (Basel, Switzerland) · 2022Review
- Supramolecular Hydrogel Based on an Osteogenic Growth Peptide Promotes Bone Defect Repair.ACS omega · 2022Article
- Effect of Inducible BMP-7 Expression on the Osteogenic Differentiation of Human Dental Pulp Stem Cells.International journal of molecular sciences · 2021Article
- Different Cell and Tissue Behavior of Micro-/Nano-Tubes and Micro-/Nano-Nets Topographies on Selective Laser Melting Titanium to Enhance Osseointegration.International journal of nanomedicine · 2021Article
- RUNX2 (6p21.1) amplification in osteosarcoma.Human pathology · 2019Article
- Human osteogenic differentiation in Space: proteomic and epigenetic clues to better understand osteoporosis.Scientific reports · 2019Article
- Multifunctional nanoparticles for intracellular drug delivery and photoacoustic imaging of mesenchymal stem cells.Drug delivery and translational research · 2019Article
- Regulation of decellularized tissue remodeling via scaffold-mediated lentiviral delivery in anatomically-shaped osteochondral constructs.Biomaterials · 2018Article
- Design and Fabrication of a Hierarchically Structured Scaffold for Tendon-to-Bone Repair.Advanced materials (Deerfield Beach, Fla.) · 2018Article
- Fabrication of 3D Scaffolds with Precisely Controlled Substrate Modulus and Pore Size by Templated-Fused Deposition Modeling to Direct Osteogenic Differentiation.Advanced healthcare materials · 2015Article
- Nanopatterning reveals an ECM area threshold for focal adhesion assembly and force transmission that is regulated by integrin activation and cytoskeleton tension.Journal of cell science · 2012Article
- Strategies for controlled delivery of growth factors and cells for bone regeneration.Advanced drug delivery reviews · 2012Review
- Runx2 promotes both osteoblastogenesis and novel osteoclastogenic signals in ST2 mesenchymal progenitor cells.Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA · 2012Article
- The food additive vanillic acid controls transgene expression in mammalian cells and mice.Nucleic acids research · 2012Article
- Inducible expression of Runx2 results in multiorgan abnormalities in mice.Journal of cellular biochemistry · 2011Article
- Mesenchymal stem cells at the intersection of cell and gene therapy.Expert opinion on biological therapy · 2010Review
- Colonization and osteogenic differentiation of different stem cell sources on electrospun nanofiber meshes.Tissue engineering. Part A · 2010Article
- Runx2 overexpression in bone marrow stromal cells accelerates bone formation in critical-sized femoral defects.Tissue engineering. Part A · 2010Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Ex vivo gene therapy is a promising approach to orthopedic regenerative medicine. These strategies typically focus on the constitutive overexpression of osteogenic factors to induce osteoblastic differentiation and matrix mineralization. However, the unregulated production of osteoinductive molecules has also resulted in abnormal bone formation and tumorigenesis. To address these limitations, this work describes a retroviral system to deliver the Runx2 osteoblastic transcription factor under control of the tetracycline-inducible (tet-off) promoter in primary skeletal myoblasts. Runx2 expression was tightly regulated by anhydrotetracyline (aTc) concentration in cell culture media. Osteoblastic gene expression, alkaline phosphatase activity, and matrix mineralization were also controlled by aTc in a dose-dependent manner. Additionally, osteoblastic differentiation was temporally regulated by adding and removing aTc from the culture media. Engineered cells were seeded onto collagen scaffolds and implanted intramuscularly in the hind limbs of syngeneic mice. In vivo mineralization by these constructs was regulated by supplementing the drinking water with aTc, as demonstrated by micro-computed tomography and histological analyses. Collectively, these results present a novel system for regulating osteoblastic differentiation of a clinically relevant autologous cell source. This system is significant to developing controlled and effective orthopedic gene therapy strategies and studying the regulation of osteoblastic differentiation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.