ArticleBritish journal of cancer2006
Multimarker RT-PCR assay for the detection of minimal residual disease in sentinel lymph nodes of breast cancer patients.
Article in British journal of cancer, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 60 citations in OpenAlex.
- Pooled it
- Three-dimensional quantitative ultrasound for detecting lymph node metastases.The Journal of surgical research · 2013Trial
- A clinical diagnostic test on the detection of sentinel lymph node metastasis in breast neoplasms using a 1-step RT-PCR.Gland surgery · 2022Article
- Detection of Minimal Residual Disease in the Peripheral Blood of Breast Cancer Patients, with a Multi Marker (MGB-1, MGB-2, CK-19 and NY-BR-1) Assay.Breast cancer (Dove Medical Press) · 2021Article
- The LINC00365/SCGB2A1 (Mammaglobin B) Axis Down-Regulates NF-κB Signaling and Is Associated with the Progression of Gastric Cancer.Cancer management and research · 2020Article
- In silico SNP analysis of the breast cancer antigen NY-BR-1.BMC cancer · 2016Article
- Novel genes associated with lymph node metastasis in triple negative breast cancer.Scientific reports · 2015Article
- One-Step Nucleic Acid Amplification in Breast Cancer Sentinel Lymph Node: A Single Institutional Experience and a Short Review.Frontiers in medicine · 2015Article
- Microarray expression profiling of dysregulated long non-coding RNAs in triple-negative breast cancer.Cancer biology & therapy · 2015Article
- Is transplantation of cryopreserved ovarian tissue from patients with advanced-stage breast cancer safe? A pilot study.Journal of assisted reproduction and genetics · 2013Article
- Pelvic lymph node dissection in the context of radical cystectomy: a thorough insight into the connection between patient, surgeon, pathologist and treating institution.Research and reports in urology · 2013Article
- Mammaglobin B (SCGB2A1) is a novel tumour antigen highly differentially expressed in all major histological types of ovarian cancer: implications for ovarian cancer immunotherapy.British journal of cancer · 2013Article
- Fine-needle aspirates CYFRA 21-1 is a useful tumor marker for detecting axillary lymph node metastasis in breast cancer patients.PloS one · 2013Article
- Accurate and rapid novel genetic diagnosis for detection of sentinel lymph node metastasis in breast cancer patients.British journal of cancer · 2012Article
- Implementing sentinel lymph node biopsy programs in developing countries: challenges and opportunities.World journal of surgery · 2011Review
- Technical limits of comparison of step-sectioning,immunohistochemistry and RT-PCR on breast cancer sentinel nodes: a study on methacarn-fixed tissue.Journal of cellular and molecular medicine · 2009Article
- Mammaglobin B is an independent prognostic marker in epithelial ovarian cancer and its expression is associated with reduced risk of disease recurrence.BMC cancer · 2009Article
- Identification of circulating tumour cells in early stage breast cancer patients using multi marker immunobead RT-PCR.Journal of hematology & oncology · 2009Article
- One-step nucleic acid amplification-a molecular method for the detection of lymph node metastases in breast cancer patients; results of the German study group.Virchows Archiv : an international journal of pathology · 2009Article
- Sentinel lymph node as a target of molecular diagnosis of lymphatic micrometastasis and local immunoresponse to malignant cells.Cancer science · 2008Review
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Authors and funding
11 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The presence of metastases in lymph nodes is the most powerful prognostic factor in breast cancer patients. Routine histological examination of lymph nodes has limited sensitivity for the detection of breast cancer metastases. The aim of the present study was to develop a multimarker reverse transcriptase-polymerase chain reaction (RT-PCR) assay for the detection of minimal residual disease in sentinel nodes of breast cancer patients. RNA was extracted from 30 sentinel lymph nodes (SLN) obtained from 28 patients, three primary breast cancers (positive controls), three lymph nodes from patients with benign diseases, and peripheral blood lymphocytes of 10 healthy volunteers (negative controls). RT-PCR was performed using the following markers; cytokeratin (CK)-19, NY-BR-1 and mammaglobin B. RT-PCR results were compared to enhanced histopathologic examination and immunohistochemistry (IHC). All three positive controls showed strong PCR amplification for all three markers. None of the 13 negative controls was amplified by any of the three markers. Among the 30 SLN analysed, breast cancer metastases were detected in six SLNs by routine histology, in eight by IHC and in 15 by RT-PCR. We conclude that a multimarker RT-PCR assay probing for NY-BR-1, mammaglobin-B, and CK-19 is more sensitive compared to enhanced pathologic examination. This method may prove to be of value in breast cancer staging and prognosis evaluation.
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