ArticleInfection and immunity2006
Biofilm growth increases phosphorylcholine content and decreases potency of nontypeable Haemophilus influenzae endotoxins.
Article in Infection and immunity, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
29 citing papers in PubMed, 57 citations in OpenAlex.
- Recreating chronic respiratory infectionsEuropean respiratory review : an official journal of the European Respiratory Society · 2024Review
- Article
- The Role of Non-TypeableFrontiers in cellular and infection microbiology · 2021Review
- Novel Antimicrobial Treatment Strategy Based on Drug Delivery Systems for Acute Otitis Media.Frontiers in pharmacology · 2021Review
- Translating Recent Microbiome Insights in Otitis Media into Probiotic Strategies.Clinical microbiology reviews · 2019Review
- Autoinducer 2 (AI-2) Production by Nontypeable Haemophilus influenzae 86-028NP Promotes Expression of a Predicted Glycosyltransferase That Is a Determinant of Biofilm Maturation, Prevention of Dispersal, and PersistenceInfection and immunity · 2018Article
- Phosphocholine-Modified Lipooligosaccharides ofMolecules (Basel, Switzerland) · 2018Article
- Incorporation of phosphorylcholine into the lipooligosaccharide of nontypeable Haemophilus influenzae does not correlate with the level of biofilm formation in vitro.Infection and immunity · 2014Article
- Relative contributions of lipooligosaccharide inner and outer core modifications to nontypeable Haemophilus influenzae pathogenesis.Infection and immunity · 2013Article
- Microbial modulation of host immunity with the small molecule phosphorylcholine.Infection and immunity · 2013Review
- The Haemophilus influenzae Sap transporter mediates bacterium-epithelial cell homeostasis.Infection and immunity · 2013Article
- Bacterial biofilms in the upper airway - evidence for role in pathology and implications for treatment of otitis media.Paediatric respiratory reviews · 2012Review
- The role of lipooligosaccharide phosphorylcholine in colonization and pathogenesis of Histophilus somni in cattle.Veterinary research · 2012Article
- Dps promotes survival of nontypeable Haemophilus influenzae in biofilm communities in vitro and resistance to clearance in vivo.Frontiers in cellular and infection microbiology · 2012Article
- Nontypeable Haemophilus influenzae biofilms: role in chronic airway infections.Frontiers in cellular and infection microbiology · 2012Review
- RbsB (NTHI_0632) mediates quorum signal uptake in nontypeable Haemophilus influenzae strain 86-028NP.Molecular microbiology · 2011Article
- Non-typeable Haemophilus influenzae invasion and persistence in the human respiratory tract.Frontiers in cellular and infection microbiology · 2011Review
- A genomic perspective on the potential of Actinobacillus succinogenes for industrial succinate production.BMC genomics · 2010Article
- Article
- LuxS promotes biofilm maturation and persistence of nontypeable haemophilus influenzae in vivo via modulation of lipooligosaccharides on the bacterial surface.Infection and immunity · 2009Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Nontypeable Haemophilus influenzae (NTHI) is a common respiratory commensal and opportunistic pathogen. NTHI is normally contained within the airways by host innate defenses that include recognition of bacterial endotoxins by Toll-like receptor 4 (TLR4). NTHI produces lipooligosaccharide (LOS) endotoxins which lack polymeric O side chains and which may contain host glycolipids. We recently showed that NTHI biofilms contain variants with sialylated LOS glycoforms that are essential to biofilm formation. In this study, we show that NTHI forms biofilms on epithelial cell layers. Confocal analysis revealed that sialylated variants were distributed throughout the biofilm, while variants expressing phosphorylcholine (PCho) were found within the biofilm. Consistent with this observation, PCho content of LOS purified from NTHI biofilms was increased compared to LOS from planktonic cultures. Hypothesizing that the observed changes in endotoxin composition could affect bioactivity, we compared inflammatory responses to NTHI LOS purified from biofilm and planktonic cultures. Our results show that endotoxins from biofilms induced weaker host innate responses. While we observed a minimal effect of sialylation on LOS bioactivity, there was a significant decrease in bioactivity associated with PCho substitutions. We thus conclude that biofilm growth increases the proportion of PCho+ variants in an NTHI population, resulting in a net decrease in LOS bioactivity. Thus, in addition to their well-documented resistance phenotypes, our data show that biofilm communities of NTHI bacteria contain variants that evoke less potent host responses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.