ArticleInfection and immunity2006
Biofilm formation by Moraxella catarrhalis in vitro: roles of the UspA1 adhesin and the Hag hemagglutinin.
Article in Infection and immunity, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 89 citations in OpenAlex.
- Application of a nanotechnology antimicrobial spray to prevent lower urinary tract infection: a multicenter urology trial.Journal of translational medicine · 2012Trial
- Synergistic biofilm formation by the coexistence of nontypeable Haemophilus influenzae and Moraxella catarrhalis reduces amoxicillin efficacy.BMC microbiology · 2026Article
- The Moraxella catarrhalis AdhC-FghA system is important for formaldehyde detoxification and protection against pulmonary clearance.Medical microbiology and immunology · 2024Article
- The burden of antibiotic resistance of the main microorganisms causing infections in humans - review of the literature.Journal of medicine and life · 2024Review
- Moraxella catarrhalis phase-variable loci show differences in expression during conditions relevant to disease.PloS one · 2020Article
- A predictedVirulence · 2019Article
- TheVirulence · 2019Article
- Airway Microbiota Dynamics Uncover a Critical Window for Interplay of Pathogenic Bacteria and Allergy in Childhood Respiratory Disease.Cell host & microbe · 2018Article
- Virulence factors of Moraxella catarrhalis outer membrane vesicles are major targets for cross-reactive antibodies and have adapted during evolution.Scientific reports · 2018Article
- An Acinetobacter trimeric autotransporter adhesin reaped from cells exhibits its nonspecific stickiness via a highly stable 3D structure.Scientific reports · 2016Article
- Staphylococcal Bap Proteins Build Amyloid Scaffold Biofilm Matrices in Response to Environmental Signals.PLoS pathogens · 2016Article
- Control of Biofilms with the Fatty Acid Signaling Molecule cis-2-Decenoic Acid.Pharmaceuticals (Basel, Switzerland) · 2015Review
- Adhesins Involved in Attachment to Abiotic Surfaces by Gram-Negative Bacteria.Microbiology spectrum · 2015Review
- The Autotransporter BpaB Contributes to the Virulence of Burkholderia mallei in an Aerosol Model of Infection.PloS one · 2015Article
- Characterization of BCAM0224, a multifunctional trimeric autotransporter from the human pathogen Burkholderia cenocepacia.Journal of bacteriology · 2014Article
- Moraxella catarrhalis expresses a cardiolipin synthase that impacts adherence to human epithelial cells.Journal of bacteriology · 2014Article
- Molecular mechanisms of moraxella catarrhalis-induced otitis media.Current allergy and asthma reports · 2013Review
- Development of a LacZ-based transcriptional reporter system for use with Moraxella catarrhalis.Plasmid · 2013Article
- Bacterial biofilms in the upper airway - evidence for role in pathology and implications for treatment of otitis media.Paediatric respiratory reviews · 2012Review
- Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Mutant analysis was used to identify Moraxella catarrhalis gene products necessary for biofilm development in a crystal violet-based assay involving 24-well tissue culture plates. The wild-type M. catarrhalis strains that formed the most extensive biofilms in this system proved to be refractory to transposon mutagenesis, so an M. catarrhalis strain was constructed that was both able to form biofilms in vitro and amenable to transposon mutagenesis. Chromosomal DNA from the biofilm-positive strain O46E was used to transform the biofilm-negative strain O35E; transformants able to form biofilms were identified and subjected to transposon-mediated mutagenesis. Biofilm-negative mutants of these transformants were shown to have a transposon insertion in the uspA1 gene. Nucleotide sequence analysis revealed that the biofilm-positive transformant T14 contained a hybrid O46E-O35E uspA1 gene, with the N-terminal 155 amino acids being derived from the O46E UspA1 protein. Transformant T14 was also shown to be unable to express the Hag protein, which normally extends from the surface of the M. catarrhalis cell. Introduction of a wild-type O35E hag gene into T14 eliminated its ability to form a biofilm. When the hybrid O46E-O35E uspA1 gene from T14 was used to replace the uspA1 gene of O35E, this transformant strain did not form a biofilm. However, inactivation of the hag gene did allow biofilm formation by strain O35E expressing the hybrid O46E-O35E uspA1 gene product. The Hag protein was shown to have an inhibitory or negative effect on biofilm formation by these M. catarrhalis strains in the crystal violet-based assay.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.