ArticleRNA (New York, N.Y.)2006
Ligation of the hairpin ribozyme in cis induced by freezing and dehydration.
Article in RNA (New York, N.Y.), 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 42 citations in OpenAlex.
- Exploring the Potential and Advancements of Circular RNA Therapeutics.Exploration (Beijing, China) · 2025Review
- Engineering of Small Ribozymes Acting on RNA: What is Needed to Make a New Function Work with an Existing Catalyst?Chembiochem : a European journal of chemical biology · 2025Review
- Review
- RT-qPCR investigation of post-mortem tissues during COVID-19.Journal of applied biomedicine · 2024Article
- A comparative survey of the influence of small self-cleaving ribozymes on gene expression in human cell culture.RNA biology · 2024Article
- Enhanced Ribozyme-Catalyzed Recombination and Oligonucleotide Assembly in Peptide-RNA Condensates.Angewandte Chemie (International ed. in English) · 2021Article
- Heat flows in rock cracks naturally optimize salt compositions for ribozymes.Nature chemistry · 2021Article
- Primitive Compartmentalization for the Sustainable Replication of Genetic Molecules.Life (Basel, Switzerland) · 2021Review
- Circular RNA: BiosynthesisFrontiers in bioengineering and biotechnology · 2021Review
- Nucleic Acid Catalysis under Potential Prebiotic Conditions.Chemistry, an Asian journal · 2020Review
- Prebiotic Factors Influencing the Activity of a Ligase Ribozyme.Life (Basel, Switzerland) · 2017Article
- Ligation of RNA Oligomers by the Schistosoma mansoni Hammerhead Ribozyme in Frozen Solution.Journal of molecular evolution · 2016Article
- Freeze-thaw cycles as drivers of complex ribozyme assembly.Nature chemistry · 2015Article
- RNA circularization strategies in vivo and in vitro.Nucleic acids research · 2015Review
- Protocells: at the interface of life and non-life.Life (Basel, Switzerland) · 2015Article
- An efficient method for long-term room temperature storage of RNA.European journal of human genetics : EJHG · 2014Article
- Circularity and self-cleavage as a strategy for the emergence of a chromosome in the RNA-based protocell.Biology direct · 2013Article
- The RNA world hypothesis: the worst theory of the early evolution of life (except for all the others)(a).Biology direct · 2012Article
- Computer simulation on the cooperation of functional molecules during the early stages of evolution.PloS one · 2012Article
- The dawn of the RNA World: toward functional complexity through ligation of random RNA oligomers.RNA (New York, N.Y.) · 2009Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although reducing the temperature slows most chemical reactions, freezing can stimulate some reactions by mechanisms that are only partially understood. Here we show that freezing stimulates the self-ligation (circularization) of linear forms of the hairpin ribozyme (HPR) containing 2',3'-cyclic phosphate and 5'-OH termini. Divalent metal ions (M2+) are not required, but monovalent cations and anions at millimolar concentrations can have various effects on this reaction depending on the specific ion. Under optimal conditions, the observed rate of M2+-independent self-ligation reaches a peak (0.04 min(-1)) at -10 degrees C with a yield of -60% after 1 h. In contrast, no ligation occurs either at above 0 degrees C or in solutions that remain unfrozen when supercooled to subzero temperatures. Under freezing conditions, the cleavage-ligation equilibrium strongly favors ligation. Besides freezing, evaporation of the aqueous solvent as well as the presence of ethanol at levels of 40% or above can also induce M2+-independent HPR ligation at 25 degrees C. We argue that partial RNA dehydration, which is a common feature of freezing, evaporation, and the presence of ethanol, is a key factor supporting HPR ligation activity at both above- and below-freezing temperatures. In the context of the RNA world hypothesis, freezing-induced ligation is an attractive mechanism by which complex RNAs could have evolved under conditions in which RNA was relatively protected against degradation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.