Evidence map›Paper›PMID 16489232›Full record

ArticleGenetics2006

Alignment of the genomes of Brachypodium distachyon and temperate cereals and grasses using bacterial artificial chromosome landing with fluorescence in situ hybridization.

Robert Hasterok, Agnieszka Marasek, Iain S Donnison, Ian Armstead, Ann Thomas, Ian P King, Elzbieta Wolny, Dominika Idziak, John Draper, Glyn Jenkins

Open access · greenAbstract read
In one paragraph

Article in Genetics, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.

0numbers the graph read from it
0cells of the map it votes in
47citing papers in PubMed
16.6field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

47 citing papers in PubMed, 113 citations in OpenAlex.

  1. Article
  2. Review
  3. Comparatively Barcoded Chromosomes ofInternational journal of molecular sciences · 2019
    Article
  4. Article
  5. The Plant cell · 2018
    Review
  6. Article
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  8. Article
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  14. Integration of Lupinus angustifolius L. (narrow-leafed lupin) genome maps and comparative mapping within legumes.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2016
    Article
  15. Article
  16. Collinearity of homoeologous group 3 chromosomes in the genus Hordeum and Secale cereale as revealed by 3H-derived FISH analysis.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2016
    Article
  17. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

Robert HasterokDepartment of Plant Anatomy and Cytology, Faculty of Biology and Environmental Protection, University of Silesia, Katowice, Poland. hasterok@us.edu.pl
Agnieszka Marasek
Iain S Donnison
Ian Armstead
Ann Thomas
Ian P King
Elzbieta Wolny
Dominika Idziak
John Draper
Glyn Jenkins
Aberystwyth University · GBUniversity of Silesia in Katowice · PLResearch Institute of Pomology and Floriculture · PL

Funding

Biotechnology and Biological Sciences Research Council BBS/E/G/00003001
6 · The paper itself

Abstract

As part of an initiative to develop Brachypodium distachyon as a genomic "bridge" species between rice and the temperate cereals and grasses, a BAC library has been constructed for the two diploid (2n = 2x = 10) genotypes, ABR1 and ABR5. The library consists of 9100 clones, with an approximate average insert size of 88 kb, representing 2.22 genome equivalents. To validate the usefulness of this species for comparative genomics and gene discovery in its larger genome relatives, the library was screened by PCR using primers designed on previously mapped rice and Poaceae sequences. Screening indicated a degree of synteny between these species and B. distachyon, which was confirmed by fluorescent in situ hybridization of the marker-selected BACs (BAC landing) to the 10 chromosome arms of the karyotype, with most of the BACs hybridizing as single loci on known chromosomes. Contiguous BACs colocalized on individual chromosomes, thereby confirming the conservation of genome synteny and proving that B. distachyon has utility as a temperate grass model species alternative to rice.

Indexed as

Chromosomes, Artificial, BacterialChromosomes, PlantEdible GrainGenetic MarkersGenome, PlantIn Situ Hybridization, FluorescenceOryzaPhysical Chromosome MappingPoaceaeQuality ControlGenetic Markers

Identifiers

PMID16489232
PMCPMC1461447
OpenAlexW2091838350

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.