ArticlePlant physiology2006
Mutations in the NB-ARC domain of I-2 that impair ATP hydrolysis cause autoactivation.
Article in Plant physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 130 papers.
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Who cites it
130 citing papers in PubMed, 311 citations in OpenAlex.
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- Functional Dissection of a Wheat NLR Protein Reveals a Minimal Active Region and Key Regulatory Sites for Immune Signalling.Molecular plant pathology · 2026Article
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- A CNL protein forms an NLR pair with NRCX to modulate plant immunity.Stress biology · 2025Article
- Molecular Mechanisms of Potato Plant-Virus-Vector Interactions.Plants (Basel, Switzerland) · 2025Review
- Assessment of Self-Activation and Inhibition of Wheat Coiled-Coil Domain Containing NLR Immune Receptor Yr10Plants (Basel, Switzerland) · 2025Article
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- Discovery of a major QTL for resistance to the guava root-knot nematode (Meloidogyne enterolobii) in 'Tanzania', an African landrace sweetpotato (Ipomoea batatas).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
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- Activation and Autoinhibition Mechanisms of NLR Immune Receptor Pi36 in Rice.International journal of molecular sciences · 2024Article
- Fine mapping and identification of a Fusarium wilt resistance gene FwS1 in pea.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Pathogen perception and signaling in plant immunity.The Plant cell · 2024Review
- Rice NLR protein XinN1, induced by a pattern recognition receptor XA21, confers enhanced resistance to bacterial blight.Plant cell reports · 2024Article
- NLR- andPlants (Basel, Switzerland) · 2023Review
- A plasma membrane nucleotide-binding leucine-rich repeat receptor mediates the recognition of the Ralstonia pseudosolanacearum effector RipY in Nicotiana benthamiana.Plant communications · 2023Article
- The Hypersensitive Response to Plant Viruses.Viruses · 2023Review
70 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Resistance (R) proteins in plants confer specificity to the innate immune system. Most R proteins have a centrally located NB-ARC (nucleotide-binding adaptor shared by APAF-1, R proteins, and CED-4) domain. For two tomato (Lycopersicon esculentum) R proteins, I-2 and Mi-1, we have previously shown that this domain acts as an ATPase module that can hydrolyze ATP in vitro. To investigate the role of nucleotide binding and hydrolysis for the function of I-2 in planta, specific mutations were introduced in conserved motifs of the NB-ARC domain. Two mutations resulted in autoactivating proteins that induce a pathogen-independent hypersensitive response upon expression in planta. These mutant forms of I-2 were found to be impaired in ATP hydrolysis, but not in ATP binding, suggesting that the ATP- rather than the ADP-bound state of I-2 is the active form that triggers defense signaling. In addition, upon ADP binding, the protein displayed an increased affinity for ADP suggestive of a change of conformation. Based on these data, we propose that the NB-ARC domain of I-2, and likely of related R proteins, functions as a molecular switch whose state (on/off) depends on the nucleotide bound (ATP/ADP).
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