ArticlePlant physiology2006
A reassessment of substrate specificity and activation of phytochelatin synthases from model plants by physiologically relevant metals.
Article in Plant physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 46 citations in OpenAlex.
- Ancient duplication and functional differentiation of phytochelatin synthases is conserved in plant genomes.Horticulture research · 2025Article
- Transcriptomics, proteomics, and metabolomics interventions prompt crop improvement against metal(loid) toxicity.Plant cell reports · 2024Review
- Effects of Inoculation with Stress-Tolerant Rhizobia on the Response of Alfalfa (Plants (Basel, Switzerland) · 2023Article
- Phytochelatins: Sulfur-Containing Metal(loid)-Chelating Ligands in Plants.International journal of molecular sciences · 2023Review
- Mechanism of calcium signal response to cadmium stress in duckweed.Plant signaling & behavior · 2022Article
- Article
- Heterologous Expression of the Phytochelatin SynthaseInternational journal of environmental research and public health · 2022Article
- Ancestral function of the phytochelatin synthase C-terminal domain in inhibition of heavy metal-mediated enzyme overactivation.Journal of experimental botany · 2020Article
- Sulfur Transport and Metabolism in Legume Root Nodules.Frontiers in plant science · 2018Review
- Tentative identification of the second substrate binding site in Arabidopsis phytochelatin synthase.PloS one · 2013Article
- Effect of magnetic nanoparticles on tobacco BY-2 cell suspension culture.International journal of environmental research and public health · 2012Article
- Characterization of the phytochelatin synthase of Schistosoma mansoni.PLoS neglected tropical diseases · 2011Article
- Characterization of the Sesbania rostrata phytochelatin synthase gene: alternative splicing and function of four isoforms.International journal of molecular sciences · 2009Article
- Chronic renal failure among farm families in cascade irrigation systems in Sri Lanka associated with elevated dietary cadmium levels in rice and freshwater fish (Tilapia).Environmental geochemistry and health · 2008Article
- Functional characterization of an unusual phytochelatin synthase, LjPCS3, of Lotus japonicus.Plant physiology · 2008Article
- Inferring the geometry of fourth-period metallic elements in arabidopsis thaliana seeds using synchrotron-based multi-angle X-ray fluorescence mapping.Annals of botany · 2007Article
- Phytochelatin synthases of the model legume Lotus japonicus. A small multigene family with differential response to cadmium and alternatively spliced variants.Plant physiology · 2007Article
- Plant nutrition challenges for a sustainable agriculture of the future.Physiologia plantarumReview
- Boosting Drought Resilience in Rice: The Priming Effects of Zaxinone and Its Mimics.Physiologia plantarumArticle
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phytochelatin synthases (PCS) catalyze phytochelatin (PC) synthesis from glutathione (GSH) in the presence of certain metals. The resulting PC-metal complexes are transported into the vacuole, avoiding toxic effects on metabolism. Legumes have the unique capacity to partially or completely replace GSH by homoglutathione (hGSH) and PCs by homophytochelatins (hPCs). However, the synthesis of hPCs has received little attention. A search for PCS genes in the model legume Lotus (Lotus japonicus) resulted in the isolation of a cDNA clone encoding a protein (LjPCS1) highly homologous to a previously reported homophytochelatin synthase (hPCS) of Glycine max (GmhPCS1). Recombinant LjPCS1 and Arabidopsis (Arabidopsis thaliana) PCS1 (AtPCS1) were affinity purified and their polyhistidine-tags removed. AtPCS1 catalyzed hPC synthesis from hGSH alone at even higher rates than did LjPCS1, indicating that GmhPCS1 is not a genuine hPCS and that a low ratio of hPC to PC synthesis is an inherent feature of PCS1 enzymes. For both enzymes, hGSH is a good acceptor, but a poor donor, of gamma-glutamylcysteine units. Purified AtPCS1 and LjPCS1 were activated (in decreasing order) by Cd2+, Zn2+, Cu2+, and Fe3+, but not by Co2+ or Ni2+, in the presence of 5 mm GSH and 50 microm metal ions. Activation of both enzymes by Fe3+ was proven by the complete inhibition of PC synthesis by the iron-specific chelator desferrioxamine. Plants of Arabidopsis and Lotus accumulated (h)PCs only in response to a large excess of Cu2+ and Zn2+, but to a much lower extent than did with Cd2+, indicating that (h)PC synthesis does not significantly contribute in vivo to copper, zinc, and iron detoxification.
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