ArticlePlant physiology2006
A transcriptome-based characterization of habituation in plant tissue culture.
Article in Plant physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 103 citations in OpenAlex.
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- Seed germination: influence of non-traditional regulating factors.Communicative & integrative biology · 2025Review
- Inducible Pluripotent Suspension Cell Cultures (iPSCs) to Study Plant Cell Differentiation.Methods in molecular biology (Clifton, N.J.) · 2024Article
- An ortholog of the MADS-box gene SEPALLATA3 regulates stamen development in the woody plant Jatropha curcas.Planta · 2022Article
- An insight into understanding the coupling between homologous recombination mediated DNA repair and chromatin remodeling mechanisms in plant genome: an update.Cell cycle (Georgetown, Tex.) · 2021Review
- Understanding In Vitro Tissue Culture-Induced Variation Phenomenon in Microspore System.International journal of molecular sciences · 2021Review
- Application of Plant Growth Regulators Modulates the Profile of Chlorogenic Acids in CulturedPlants (Basel, Switzerland) · 2021Article
- Transcriptional memories mediate the plasticity of cold stress responses to enable morphological acclimation in Brachypodium distachyon.The New phytologist · 2021Article
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- Elevation of secondary metabolites production through light-emitting diodes (LEDs) illumination in protocorm-like bodies (PLBs) ofBiotechnology reports (Amsterdam, Netherlands) · 2020Article
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- Auxin decreases chromatin accessibility through the TIR1/AFBs auxin signaling pathway in proliferative cells.Scientific reports · 2018Article
- The ISWI remodeler in plants: protein complexes, biochemical functions, and developmental roles.Chromosoma · 2017Review
- Analysis of CFB, a cytokinin-responsive gene of Arabidopsis thaliana encoding a novel F-box protein regulating sterol biosynthesis.Journal of experimental botany · 2017Article
- Inheritance of vernalization memory at FLOWERING LOCUS C during plant regeneration.Journal of experimental botany · 2017Article
- Hormonal and epigenetic regulation during embryogenic tissue habituation in Cucurbita pepo L.Plant cell reports · 2016Article
- Transition from somatic embryo to friable embryogenic callus in cassava: dynamic changes in cellular structure, physiological status, and gene expression profiles.Frontiers in plant science · 2015Article
- Using a periclinal chimera to unravel layer-specific gene expression in plants.The Plant journal : for cell and molecular biology · 2013Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For the last 50 years, scientists have recognized that varying ratios of the plant hormones cytokinin and auxin induce plant cells to form particular tissues: undifferentiated calli, shoot structures, root structures, or a whole plant. Proliferation of undifferentiated callus tissue, greening, and the formation of shoot structures are all cytokinin-dependent processes. Habituation refers to a naturally occurring phenomenon whereby callus cultures, upon continued passage, lose their requirement for cytokinin. Earlier studies of calli with a higher-than-normal cytokinin content indicate that overproduction of cytokinin by the culture tissues is a possible explanation for this acquired cytokinin independence. A transcriptome-based analysis of a well established habituated Arabidopsis (Arabidopsis thaliana) cell culture line was undertaken, to explore genome-wide expression changes underlying the phenomenon of habituation. Increased levels of expression of the cytokinin receptor CRE1, as well as altered levels of expression of several other genes involved in cytokinin signaling, indicated that naturally acquired deregulation of cytokinin-signaling components could play a previously unrecognized role in habituation. Up-regulation of several cytokinin oxidases, down-regulation of several known cytokinin-inducible genes, and a lack of regulation of the cytokinin synthases indicated that increases in hormone concentration may not be required for habituation. In addition, up-regulation of the homeodomain transcription factor FWA, transposon-related elements, and several DNA- and chromatin-modifying enzymes indicated that epigenetic changes contribute to the acquisition of cytokinin habituation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.