ArticleCurrent biology : CB2006
Rho-kinase controls cell shape changes during cytokinesis.
Article in Current biology : CB, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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48 citing papers in PubMed, 90 citations in OpenAlex.
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- Myosin light chain kinase-driven myosin II turnover regulates actin cortex contractility during mitosis.Molecular biology of the cell · 2021Article
- CLIC4 is a cytokinetic cleavage furrow protein that regulates cortical cytoskeleton stability during cell division.Journal of cell science · 2020Article
- Adherens junction remodelling during mitotic rounding of pseudostratified epithelial cells.EMBO reports · 2020Article
- Rocking the Boat: The Decisive Roles of Rho Kinases During Oocyte, Blastocyst, and Stem Cell Development.Frontiers in cell and developmental biology · 2020Review
- Animal Cell Cytokinesis: The Rho-Dependent Actomyosin-Anilloseptin Contractile Ring as a Membrane Microdomain Gathering, Compressing, and Sorting Machine.Frontiers in cell and developmental biology · 2020Article
- Rho-dependent control of the Citron kinase, Sticky, drives midbody ring maturation.Molecular biology of the cell · 2019Article
- Asymmetric recruitment and actin-dependent cortical flows drive the neuroblast polarity cycle.eLife · 2019Article
- Dynamic blebbing: A bottleneck to human embryonic stem cell culture that can be overcome by Laminin-Integrin signaling.Stem cell research · 2018Article
- A positive-feedback-based mechanism for constriction rate acceleration during cytokinesis ineLife · 2018Article
- Cell-cell adhesion accounts for the different orientation of columnar and hepatocytic cell divisions.The Journal of cell biology · 2017Article
- Myosin efflux promotes cell elongation to coordinate chromosome segregation with cell cleavage.Nature communications · 2017Article
- Kinase signaling and targeted therapy for primary myelofibrosis.Experimental hematology · 2017Review
- Coupling changes in cell shape to chromosome segregation.Nature reviews. Molecular cell biology · 2016Review
- Drak Is Required for Actomyosin Organization During Drosophila Cellularization.G3 (Bethesda, Md.) · 2016Article
- Augmin shapes the anaphase spindle for efficient cytokinetic furrow ingression and abscission.Molecular biology of the cell · 2016Article
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundAnimal cell cytokinesis is characterized by a sequence of dramatic cortical rearrangements. How these are coordinated and coupled with mitosis is largely unknown. To explore the initiation of cytokinesis, we focused on the earliest cell shape change, cell elongation, which occurs during anaphase B and prior to cytokinetic furrowing.
resultsUsing RNAi and live video microscopy in Drosophila S2 cells, we implicate Rho-kinase (Rok) and myosin II in anaphase cell elongation. rok RNAi decreased equatorial myosin II recruitment, prevented cell elongation, and caused a remarkable spindle defect where the spindle poles collided with an unyielding cell cortex and the interpolar microtubules buckled outward as they continued to extend. Disruption of the actin cytoskeleton with Latrunculin A, which abolishes cortical rigidity, suppressed the spindle defect. rok RNAi also affected furrowing, which was delayed and slowed, sometimes distorted, and in severe cases blocked altogether. Codepletion of the myosin binding subunit (Mbs) of myosin phosphatase, an antagonist of myosin II activation, only partially suppressed the cell-elongation defect and the furrowing delay, but prevented cytokinesis failures induced by prolonged rok RNAi. The marked sensitivity of cell elongation to Rok depletion was highlighted by RNAi to other genes in the Rho pathway, such as pebble, racGAP50C, and diaphanous, which had profound effects on furrowing but lesser effects on elongation.
conclusionsWe show that cortical changes underlying cell elongation are more sensitive to depletion of Rok and myosin II, in comparison to other regulators of cytokinesis, and suggest that a distinct regulatory pathway promotes cell elongation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.