ArticleJournal of clinical pathology1991
Detection of single copies of Epstein-Barr virus in paraffin wax sections by non-radioactive in situ hybridisation.
Article in Journal of clinical pathology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 26 citations in OpenAlex.
- Detection of Epstein-Barr virus genomes in peripheral blood B cells from solid-organ transplant recipients by fluorescence in situ hybridization.Journal of clinical microbiology · 2002Article
- Assessment of the prognostic impact of the Epstein-Barr virus-encoded latent membrane protein-1 expression in Hodgkin's disease.British journal of cancer · 2001Article
- Demystified ... in situ hybridisation.Molecular pathology : MP · 1998Review
- DNA viruses in the pathogenesis of sporadic chronic idiopathic intestinal pseudo-obstruction.Gut · 1997Article
- Chemiluminescent in situ hybridization for the detection of cytomegalovirus DNA.The American journal of pathology · 1996Article
- Detection of Epstein-Barr virus in a case of undifferentiated nasopharyngeal carcinoma by in situ hybridization with digoxigenin-labelled PCR-generated probes.Virchows Archiv. A, Pathological anatomy and histopathology · 1993Article
- Epstein Barr virus (EBV) encoded small RNAs: targets for detection by in situ hybridisation with oligonucleotide probes.Journal of clinical pathology · 1992Article
- Presence of Epstein-Barr virus in Hodgkin's disease is not exclusive to Reed-Sternberg cells.The American journal of pathology · 1992Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A highly sensitive non-isotopic in situ hybridisation technique was developed for the localisation of Epstein-Barr virus (EBV) in paraffin wax embedded tissue sections. The method uses a repeated sequence of the EBV genome as a probe, labelled with the novel reporter molecule, digoxigenin. The method can identify individual copies of EBV by detection of both EBV DNA and highly localised RNA transcripts. A combination of careful proteolytic digestion of tissue sections, high temperature denaturation of probe and target DNA, and sensitive immunocytochemical detection are used to attain single copy sensitivity. The technique is quicker and simpler to perform than some other methods used for the identification of EBV, and provides simultaneous morphological information which cannot be obtained by methods using tissue extracts. This method permits the investigation of the role of EBV in neoplastic conditions of lymphoid and epithelial cells, and may prove valuable in determining the sites of latent virus in healthy subjects.
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